Itga8^tm1Lfr
Targeted Allele Detail

Summary

• Symbol: Itga8^tm1Lfr
• Name: integrin alpha 8; targeted mutation 1, Louis F Reichardt
• MGI ID: MGI:2181699
• Synonyms: alpha8-
• Gene: Itga8 Location: Chr2:12111443-12306733 bp, - strand Genetic Position: Chr2, 9.12 cM
• Alliance: Itga8^tm1Lfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38838
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The exon encoding amino acids 362-424 was disrupted by the insertion of a neomycin selection cassette. Western blot analysis of kidney tissue, using an antibody raised against the extracellular domain, showed an absence of encoded protein in homozygous mutant mice. The protein was also shown to be absent in lung tissue via immunohistochemical analysis. (J:38838)

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Itga8 Mutation: 74 strains or lines available

References

• Original: J:38838 Muller U, et al., Integrin alpha8beta1 is critically important for epithelial-mesenchymal interactions during kidney morphogenesis. Cell. 1997 Mar 7;88(5):603-13
• All: 12 reference(s)