Cd9^tm1Mek
Targeted Allele Detail

Summary

• Symbol: Cd9^tm1Mek
• Name: CD9 antigen; targeted mutation 1, Eisuke Mekada
• MGI ID: MGI:2181695
• Synonyms: CD9^-
• Gene: Cd9 Location: Chr6:125437229-125471723 bp, - strand Genetic Position: Chr6, 59.32 cM
• Alliance: Cd9^tm1Mek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59402
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence, encompassing a portion of exon 3 and all of exon 4 and encoding the second and third transmembrane domains, was replaced with a neomycin selection cassette. Flow cytometric analysis showed an absence of encoded protein on the surface of bone marrow cells obtained from homozygous mutant mice. An absence of protein expression on homozygous mutant oocyte plasma membranes was determined via immunofluorescent staining. (J:59402)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd9 Mutation: 103 strains or lines available

References

• Original: J:59402 Miyado K, et al., Requirement of CD9 on the egg plasma membrane for fertilization. Science. 2000 Jan 14;287(5451):321-4
• All: 26 reference(s)