Lipe^tm1Ishi
Targeted Allele Detail

Summary

• Symbol: Lipe^tm1Ishi
• Name: lipase, hormone sensitive; targeted mutation 1, Shun Ishibashi
• MGI ID: MGI:2181610
• Synonyms: HSL-, HSL-KO
• Gene: Lipe Location: Chr7:25078952-25097911 bp, - strand Genetic Position: Chr7, 13.78 cM, cytoband A3
• Alliance: Lipe^tm1Ishi page

Lipe^tm1Ishi/Lipe^tm1Ishi males exhibit oligospermia and seminiferous tubule defects

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59889
• Parent Cell Line: JH1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Portions of exon 5 and exon 6, as well as the intervening intron, were replaced with a neo cassette. The deleted region encoded the catalytic domain. Northern blot analysis of epididymal fat obtained from homozygous mutant mice showed a lack of normal transcript. An absence of protein in homozygous mutant mice was demonstrated by Western blot analysis of the epididymal fat and testes. Neutral cholesterol ester hydrolase (NCEH) activity was undetected by assays involving white and brown adipose tissue. (J:59889)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lipe Mutation: 36 strains or lines available

References

• Original: J:59889 Osuga J, et al., Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity [see comments]. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):787-92
• All: 20 reference(s)