Prkacb^tm1Gsm
Targeted Allele Detail

Summary

• Symbol: Prkacb^tm1Gsm
• Name: protein kinase, cAMP dependent, catalytic, beta; targeted mutation 1, G Stanley McKnight
• MGI ID: MGI:2180946
• Synonyms: Cbeta1^-
• Gene: Prkacb Location: Chr3:146435334-146518691 bp, - strand Genetic Position: Chr3, 72.28 cM, cytoband H3
• Alliance: Prkacb^tm1Gsm page

Neural tube defects in Prkaca^tm1Gsm/Prkaca⁺ Prkacb^tm1Gsm/Prkacb^tm1Gsm and Prkaca^tm1Gsm/Prkaca^tm1Gsm Prkacb^tm1Gsm/Prkacb⁺ mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31731
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 200 bp region of the gene spanning amino acid 5 of exon 1 to part of intron 1 was replaced with a neomycin resistance cassette via homologous recombination. The gene targeting event specifically disrupts the beta 1 isoform of the gene product. The beta 2 and beta 3 isoforms, which do not utilize exon 1, remain intact. Western blot analysis verified the absence of beta 1 isoform in homozygous mutant animals. (J:31731)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkacb Mutation: 32 strains or lines available

Notes

ES cell line = REK2 or REK3.

References

• Original: J:31731 Qi M, et al., Impaired hippocampal plasticity in mice lacking the Cbeta1 catalytic subunit of cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1571-6
• All: 6 reference(s)