Cd3g^tm1Amk
Targeted Allele Detail

Summary

• Symbol: Cd3g^tm1Amk
• Name: CD3 antigen, gamma polypeptide; targeted mutation 1, Ada M Kruisbeek
• MGI ID: MGI:2180763
• Synonyms: CD3gamma^-
• Gene: Cd3g Location: Chr9:44880870-44891729 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3g^tm1Amk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47013
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4, which encodes the transmembrane domain, was replaced with a neomycin cassette. In addition a frameshift mutation was introduced into exon 3. Northern blot analysis of homozygous mutant thymocytes showed an absence of normal transcript. The analysis also showed that the expression of the closely related CD3 antigen genes, Cd3d and Cd3g, was not affected in mutant mice. The levels of Cd3z transcript, however, were found to be slightly lower than in wild-type. (J:47013)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd3g Mutation: 24 strains or lines available

References

• Original: J:47013 Haks MC, et al., The CD3gamma chain is essential for development of both the TCRalphabeta and TCRgamma- delta lineages. EMBO J. 1998 Apr 1;17(7):1871-82
• All: 13 reference(s)