Rev3l^tm1Kaji
Targeted Allele Detail

Summary

• Symbol: Rev3l^tm1Kaji
• Name: REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1, Kagemasa Kajiwara
• MGI ID: MGI:2178815
• Synonyms: Sez4-
• Gene: Rev3l Location: Chr10:39608114-39751207 bp, + strand Genetic Position: Chr10, 21.04 cM
• Alliance: Rev3l^tm1Kaji page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74111
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Amino acids 1327 - 1336 of this gene were replaced by a PGK-neo-PolyA cassette via homologous recombination in the orientation of gene transcription. A stop codon engineered into the 5' end of the neo cassette results in the loss of the DNA polymerase domain of the targeted gene. Southern blot analysis confirmed successful gene targeting in ES cells. (J:74111)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rev3l Mutation: 118 strains or lines available

References

• Original: J:74111 Kajiwara K, et al., Sez4 gene encoding an elongation subunit of DNA polymerase zeta is required for normal embryogenesis. Genes Cells. 2001 Feb;6(2):99-106
• All: 2 reference(s)