Gria1^tm1Erk
Targeted Allele Detail

Summary

• Symbol: Gria1^tm1Erk
• Name: glutamate receptor, ionotropic, AMPA1 (alpha 1); targeted mutation 1, Esa R Korpi
• MGI ID: MGI:2178080
• Synonyms: GluR-A^R
• Gene: Gria1 Location: Chr11:56902342-57221070 bp, + strand Genetic Position: Chr11, 34.51 cM
• Alliance: Gria1^tm1Erk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75498
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Single point mutation
• Mutation details: A single loxP site was inserted into intron 10 and a loxP flanked neomycin selection cassette was inserted into exon 11. In addition, a point mutation was introduced into exon 11 that altered codon 582 from one encoding glutamine to one encoding arginine. The neomycin resistance cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice, leaving loxP sites flanking the mutated exon 11 in the final, heritable allele. RT-PCR analysis demonstrated that a transcript was produced from this allele at normal expression levels, and western blot analysis confirmed the expression of a mutant protein. (J:75498)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gria1 Mutation: 62 strains or lines available

References

• Original: J:75498 Vekovischeva OY, et al., Morphine-induced dependence and sensitization are altered in mice deficient in AMPA-type glutamate receptor-A subunits. J Neurosci. 2001 Jun 15;21(12):4451-9
• All: 4 reference(s)