Hoxd12^tm2Ddu
Targeted Allele Detail

Summary

• Symbol: Hoxd12^tm2Ddu
• Name: homeobox D12; targeted mutation 2, Denis Duboule
• MGI ID: MGI:2177960
• Synonyms: Hoxd-12^lac
• Gene: Hoxd12 Location: Chr2:74505357-74508049 bp, + strand Genetic Position: Chr2, 44.13 cM
• Alliance: Hoxd12^tm2Ddu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36770
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ reporter was inserted in frame with the Hoxd12 gene, at the StyI site of the first exon. In addition, a loxP-PGKneo-loxP cassette was inserted next to the lacZ cassette, in the same transcriptional orientation. Excision of the selectable marker was conducted and the resultant clones were injected into blastocysts and transferred to the germ line. The resultant targeted allele had no deletion of genomic sequences. (J:36770)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hoxd12 Mutation: 13 strains or lines available

References

• Original: J:36770 Kondo T, et al., Function of posterior HoxD genes in the morphogenesis of the anal sphincter. Development. 1996 Sep;122(9):2651-9
• All: 3 reference(s)