Eng^tm1Hma
Targeted Allele Detail

Summary

• Symbol: Eng^tm1Hma
• Name: endoglin; targeted mutation 1, Helen M Arthur
• MGI ID: MGI:2177822
• Synonyms: Eng^-
• Gene: Eng Location: Chr2:32536607-32572681 bp, + strand Genetic Position: Chr2, 22.09 cM
• Alliance: Eng^tm1Hma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73415
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An HA tag followed by a stop codon was introduced into exon 8, and was followed by an IRES-lacZ gene and neomycin selection cassette that replaced exons 9, 10 and 11. Western blot analysis on homozygous embryos demonstrated that no detectable ENG protein was expressed from this allele; however, a beta-galactosidase protein was expressed under the control of the endogenous promoter. (J:73415)

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eng Mutation: 41 strains or lines available

References

• Original: J:73415 Arthur HM, et al., Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development. Dev Biol. 2000 Jan 1;217(1):42-53
• All: 15 reference(s)