Gabra1^tm1Rmck
Targeted Allele Detail

Summary

• Symbol: Gabra1^tm1Rmck
• Name: gamma-aminobutyric acid type A receptor subunit alpha 1; targeted mutation 1, Ruth M McKernan
• MGI ID: MGI:2177611
• Synonyms: alphaH101R
• Gene: Gabra1 Location: Chr11:42021766-42073757 bp, - strand Genetic Position: Chr11, 24.97 cM
• Alliance: Gabra1^tm1Rmck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62214
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A point mutation was introduced that altered codon 101 from one encoding histidine to one encoding arginine (H101R). This alteration had been shown to render the GABA-A receptor insensitive to drugs such as diazepam. A loxP flanked neomycin selection cassette was also inserted into an adjacent intron, but was removed by Cre mediated recombination in the final allele. (J:62214)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabra1 Mutation: 47 strains or lines available

References

• Original: J:62214 McKernan RM, et al., Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABA(A) receptor alpha1 subtype [see comments]. Nat Neurosci. 2000 Jun;3(6):587-92
• All: 3 reference(s)