Dmc1^tm1Jcs
Targeted Allele Detail

Summary

• Symbol: Dmc1^tm1Jcs
• Name: DNA meiotic recombinase 1; targeted mutation 1, John C Schimenti
• MGI ID: MGI:2176331
• Synonyms: Dmc1^-
• Gene: Dmc1 Location: Chr15:79445698-79489310 bp, - strand Genetic Position: Chr15, 37.79 cM, cytoband E
• Alliance: Dmc1^tm1Jcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47308
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing sequences encoding the conserved DNA binding motif required for protein activity. RT-PCR analysis on RNA derived from testis of homozygous males demonstrated that no detectable transcript was produced from this allele. (J:47308)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dmc1 Mutation: 16 strains or lines available

References

• Original: J:47308 Pittman DL, et al., Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog. Mol Cell. 1998 Apr;1(5):697-705
• All: 40 reference(s)