Plg^tm1Dco
Targeted Allele Detail

Summary

• Symbol: Plg^tm1Dco
• Name: plasminogen; targeted mutation 1, Desire Collen
• MGI ID: MGI:2158761
• Synonyms: plasminogen^-, Plg-
• Gene: Plg Location: Chr17:12597496-12638271 bp, + strand Genetic Position: Chr17, 8.5 cM
• Alliance: Plg^tm1Dco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:30174
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing exons 15-17 which encodes part of the catalytic domain. Northern blot analysis on RNA derived from liver of homozygous mice demonstrated that no detectable transcript was produced from this allele. ELISA assays on plasma derived from homozygous mice confirmed that no active protein was detectable. (J:30174)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plg Mutation: 4 strains or lines available

Notes

Homozygous mutant mice survive embryonic development, but after birth show retarded growth and reduced fertility and survival. Impaired thrombolysis and fibrin deposition are observed. The symptoms resemble those following loss of both tissue and urokinase plasminogen activation (J:30174). Treatment of null mutant mice with exogenous plasminogen restores thrombolysis (J:34531). Ligneous conjunctivitis (OMIM 217090) has also been ascribed to PLG mutations (J:48307).

References

• Original: J:30174 Ploplis VA, et al., Effects of disruption of the plasminogen gene on thrombosis, growth, and health in mice. Circulation. 1995 Nov 1;92(9):2585-93
• All: 51 reference(s)