St8sia1^tm1Rlp
Targeted Allele Detail

Summary

• Symbol: St8sia1^tm1Rlp
• Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1; targeted mutation 1, Richard L Proia
• MGI ID: MGI:2158360
• Synonyms: GD3S -, Siat8a^-
• Gene: St8sia1 Location: Chr6:142767271-142910178 bp, - strand Genetic Position: Chr6, 74.78 cM
• Alliance: St8sia1^tm1Rlp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67955
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An IRES-lacZ and neomycin selection cassette was inserted into exon 5, which encodes the sialyl S motif, an essential part of the catalytic domain of the encoded protein. TLC analysis on brain extract of homozygous mice demonstrated that no active protein was produced from this allele. (J:67955)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any St8sia1 Mutation: 28 strains or lines available

References

• Original: J:67955 Kawai H, et al., Mice expressing only monosialoganglioside GM3 exhibit lethal audiogenic seizures. J Biol Chem. 2001 Mar 9;276(10):6885-8
• All: 18 reference(s)