Col10a1^tm1Ksec
Targeted Allele Detail

Summary

• Symbol: Col10a1^tm1Ksec
• Name: collagen, type X, alpha 1; targeted mutation 1, Kathryn S E Cheah
• MGI ID: MGI:2153000
• Synonyms: Col10a-1-
• Gene: Col10a1 Location: Chr10:34265977-34273081 bp, + strand Genetic Position: Chr10, 18.85 cM, cytoband B1-B3
• Alliance: Col10a1^tm1Ksec page

Altered trabecular morphology and organization in the growth plate of Col10a1^tm1Ksec/Col10a1^tm1Ksec mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38124
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 3, disrupting sequences encoding the beginning of the helix domain. RT-PCR assays did not detect expression of a normal transcript in cartilage RNA derived from homozygous mice, although low levels of a mutant transcript were detected. Immunohistochemical analysis on cartilage of 2 day old homozygous mice confirmed the deficiency of the protein. (J:38124)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col10a1 Mutation: 27 strains or lines available

References

• Original: J:38124 Kwan KM, et al., Abnormal compartmentalization of cartilage matrix components in mice lacking collagen X: implications for function. J Cell Biol. 1997 Jan 27;136(2):459-71
• All: 4 reference(s)