Gnai2^tm1Lbi
Targeted Allele Detail

Summary

• Symbol: Gnai2^tm1Lbi
• Name: G protein subunit alpha i2; targeted mutation 1, Lutz Birnbaumer
• MGI ID: MGI:2152603
• Synonyms: alphai2-, Gai2^-, Galphai2-, Galphai2-KO, Gnai2^-, Gnai2^tm1Uru, Gnao1.2^tm1LBi
• Gene: Gnai2 Location: Chr9:107491324-107512542 bp, - strand Genetic Position: Chr9, 58.43 cM
• Alliance: Gnai2^tm1Lbi page

Rib and sternum defects in mice with mutations of Gnai1, Gnai2, and/or Gnai3

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194592
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 3. The truncated protein is predicted to be inactive and also lack the cysteine at position 352 which is ADP-ribosylated by pertussis toxin. No protein was detected in homogenates from homozygous mice by ADP-ribosylation with pertussis toxin. The absence of protein was also confirmed by immunoblotting. (J:24434, J:194592)

Disease models

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Gnai2^tm1Lbi

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gnai2 Mutation: 54 strains or lines available

References

• Original: J:194592 Rudolph U, et al., Disruption of the G(i2) alpha locus in embryonic stem cells and mice: a modified hit and run strategy with detection by a PCR dependent on gap repair. Transgenic Res. 1993 Nov;2(6):345-55
• All: 77 reference(s)