Grik2^tm1Sfh
Targeted Allele Detail

Summary

• Symbol: Grik2^tm1Sfh
• Name: glutamate receptor, ionotropic, kainate 2 (beta 2); targeted mutation 1, Stephen F Heinemann
• MGI ID: MGI:1857979
• Synonyms: GLUK6-, GluR6-
• Gene: Grik2 Location: Chr10:48970929-49664862 bp, - strand Genetic Position: Chr10, 24.87 cM
• Alliance: Grik2^tm1Sfh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46932
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a 687 bp fragment of the gene, which contained the exon encoding MD2. In situ hybridization of parasagittal sections of brains from homozygous mutant mice probed with gene-specific probes showed no detectable mRNA for the targeted gene. Immunoblots of hippocampal membrane extracts from homozygous mutant mice using antibodies against the targeted protein showed no detectable protein. (J:46932)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grik2 Mutation: 64 strains or lines available

References

• Original: J:46932 Mulle C, et al., Altered synaptic physiology and reduced susceptibility to kainate-induced seizures in GluR6-deficient mice. Nature. 1998 Apr 9;392(6676):601-5
• All: 38 reference(s)