Ikbkb^tm1Mka
Targeted Allele Detail

Summary

• Symbol: Ikbkb^tm1Mka
• Name: inhibitor of kappaB kinase beta; targeted mutation 1, Michael Karin
• MGI ID: MGI:1857769
• Synonyms: IKKbeta-
• Gene: Ikbkb Location: Chr8:23149228-23196605 bp, - strand Genetic Position: Chr8, 11.42 cM, cytoband A3-A4
• Alliance: Ikbkb^tm1Mka page

Anemic appearance of E13.5 Ikbkb^tm1Mka/Ikbkb^tm1Mka mutants

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:55737
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced part of the second and the entire third coding exon of the gene, which encodes for a part of the kinase domain of the protein. Western blots of protein extracts from embryonic fibroblasts from homozygous mutant mice showed no detectable protein for the targeted gene. (J:55737)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikbkb Mutation: 56 strains or lines available

Notes

ES cell line = GS (Genome Systems).

References

• Original: J:55737 Li ZW, et al., The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis. J Exp Med. 1999 Jun 7;189(11):1839-45
• All: 12 reference(s)