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Gene Expression Data
Assay Details
Assay
Reference: J:334114 Ruthig VA, et al., The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late gestation. PLoS Genet. 2023 Mar;19(3):e1010656
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:8260926
Gene symbol: Dnd1
Gene name: DND microRNA-mediated repression inhibitor 1
Reporter: GFP
Antibody: anti-GFP (ab13970)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S2A: embryonic day 16.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present Non-Uniform S2A , S2a' High (arrow) and low (wedge) GFP level male germ cells were found.
Specimen S2B: embryonic day 16.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present S2b' , S2B A high GFP level male germ cell.
Specimen S2C: embryonic day 16.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present S2C , S2c' A low GFP level male germ cell.
Specimen S5A: embryonic day 14.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Note: Triple labeled: green - Dnd1 reporter; blue - Gcna (assay MGI:8260927); red - ORF1p.
Structure Level Pattern Image Note
TS22: germ cell of testis Present Non-Uniform S5A , S5a'' There was no correlation of ORF1p staining with GFP reporter high or low levels.
Specimen S5B: embryonic day 14.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Note: Triple labeled: green - Dnd1 reporter; blue - Gcna (assay MGI:8260927); red - Tomm20 (assay MGI:8260928).
Structure Level Pattern Image Note
TS22: germ cell of testis Present Non-Uniform S5b'' , S5B There was no correlation of Tomm20 staining with GFP reporter high or low levels.
Specimen S5C: embryonic day 14.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Note: Triple labeled: green - Dnd1 reporter; blue - Gcna (assay MGI:8260927); red - Parp1 (assay MGI:8260929).
Structure Level Pattern Image Note
TS22: germ cell of testis Present Non-Uniform S5C , S5c'' There was no correlation of Parp1 staining with GFP reporter high or low levels.
Specimen S5D: embryonic day 18.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Note: Triple labeled: green - Dnd1 reporter; blue - Gcna (assay MGI:8260927); red - ORF1p.
Structure Level Pattern Image Note
TS26: germ cell of testis Present Non-Uniform S5D , S5d'' There was no correlation of ORF1p staining with GFP reporter high or low levels.
Specimen S5E: embryonic day 18.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Note: Triple labeled: green - Dnd1 reporter; blue - Gcna (assay MGI:8260927); red - Tomm20 (assay MGI:8260928).
Structure Level Pattern Image Note
TS26: germ cell of testis Present Non-Uniform S5E , S5e'' There was no correlation of Tomm20 staining with GFP reporter high or low levels.
Specimen S5F: embryonic day 18.5; Dnd1em1Cpl/Dnd1em1Cpl (more )
Note: Triple labeled: green - Dnd1 reporter; blue - Gcna (assay MGI:8260927); red - Parp1 (assay MGI:8260929).
Structure Level Pattern Image Note
TS26: germ cell of testis Present Non-Uniform S5F , S5f'' There was no correlation of Parp1 staining with GFP reporter high or low levels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
09/15/2026
MGI 6.29 		The Jackson Laboratory