About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:306107 Niborski LL, et al., Hnf1b haploinsufficiency differentially affects developmental target genes in a new renal cysts and diabetes mouse model. Dis Model Mech. 2021 May 1;14(5):dmm047498
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8187725
Gene symbol: Hnf4a
Gene name: hepatic nuclear factor 4, alpha
Antibody: Anti-HNF-4-alpha [EPR16885] (ab181604)
Assay notes: Embryos and postnatal kidneys up to 2 months of age were fixed with 60% ethanol/11% formaldehyde and 10% acetic acid.
Results
Specimen 3E: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 3E
Specimen 3E': embryonic day 15.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Weak Regionally restricted 3E' Decreased expression compared to wild type.
Specimen 3G: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present 3G
Specimen 3G': embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Weak 3G' Decreased expression compared to wild type.
Specimen 4B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present 4B Co-expression with Hnf1b in proximal tubules.
Specimen 4E: embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present Regionally restricted 4E Expression in dilated proximal tubules.
Specimen 4H: embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present Regionally restricted 4H Expression in dilated proximal tubules.
TS24: maturing glomerular tuft Present Regionally restricted 4H Disorganized expression in glomerular cysts.
Specimen 5A: embryonic day 15.5 (more )
Note: Double labeled: green - Hnf4a; red - Vil1 (assay MGI:8187726).
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 5A
Specimen 5B/C: embryonic day 15.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Note: Double labeled: green - Hnf4a; red - Vil1 (assay MGI:8187726).
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 5C , 5B
Specimen S5A: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: renal proximal tubule Present S5A
Specimen S5A': embryonic day 17.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS25: renal proximal tubule Weak Regionally restricted S5A' Expression decreased in unequal clusters of proximal tubules.
Specimen S5F: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: renal proximal tubule Present S5F
Specimen S5F': postnatal day 0; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS27: renal proximal tubule Weak Regionally restricted S5F' Expression decreased in unequal clusters of proximal tubules.
Specimen S6B WT: embryonic day 15.5 (more )
Note: Double labeled: red - Hnf4a; green - phosphohistone H3.
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present S6B left lower , S6B left middle , S6B WT , S6B left upper
Specimen S6B Sp2/+: embryonic day 15.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Note: Double labeled: red - Hnf4a; green - phosphohistone H3.
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present S6B right (3rd row) , S6B right upper , S6B Sp2/+ , S6B right (2nd row) , S6B right lower
Specimen S8 HNF4A WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present S8_HNF4A WT
Specimen S8 HNF4A Sp2/+: embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Weak S8_HNF4A Sp2/+ Expression strongly downregulated compared to wild type.
Specimen S8 HNF4A Hnf1a-/: embryonic day 16.5; Hnf1atm1.1Ylee/Hnf1atm1.1Ylee (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present S8_HNF4A Hnf1a-/- Expression not downregulated compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
10/06/2026
MGI 6.29 		The Jackson Laboratory