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Gene Expression Data
Assay Details
Assay
Reference: J:306107 Niborski LL, et al., Hnf1b haploinsufficiency differentially affects developmental target genes in a new renal cysts and diabetes mouse model. Dis Model Mech. 2021 May 1;14(5):dmm047498
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8187725
Gene symbol: Hnf4a
Gene name: hepatic nuclear factor 4, alpha
Antibody: Anti-HNF-4-alpha [EPR16885] (ab181604)
Assay notes: Embryos and postnatal kidneys up to 2 months of age were fixed with 60% ethanol/11% formaldehyde and 10% acetic acid.
Results
Specimen 3E: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 3E
Specimen 3E': embryonic day 15.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Weak Regionally restricted 3E' Decreased expression compared to wild type.
Specimen 3G: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present 3G
Specimen 3G': embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Weak 3G' Decreased expression compared to wild type.
Specimen 4B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present 4B Co-expression with Hnf1b in proximal tubules.
Specimen 4E: embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present Regionally restricted 4E Expression in dilated proximal tubules.
Specimen 4H: embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present Regionally restricted 4H Expression in dilated proximal tubules.
TS24: maturing glomerular tuft Present Regionally restricted 4H Disorganized expression in glomerular cysts.
Specimen 5A: embryonic day 15.5 (more )
Note: Double labeled: green - Hnf4a; red - Vil1 (assay MGI:8187726).
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 5A
Specimen 5B/C: embryonic day 15.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Note: Double labeled: green - Hnf4a; red - Vil1 (assay MGI:8187726).
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 5C , 5B
Specimen S5A: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: renal proximal tubule Present S5A
Specimen S5A': embryonic day 17.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS25: renal proximal tubule Weak Regionally restricted S5A' Expression decreased in unequal clusters of proximal tubules.
Specimen S5F: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: renal proximal tubule Present S5F
Specimen S5F': postnatal day 0; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS27: renal proximal tubule Weak Regionally restricted S5F' Expression decreased in unequal clusters of proximal tubules.
Specimen S6B WT: embryonic day 15.5 (more )
Note: Double labeled: red - Hnf4a; green - phosphohistone H3.
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present S6B left lower , S6B left middle , S6B WT , S6B left upper
Specimen S6B Sp2/+: embryonic day 15.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Note: Double labeled: red - Hnf4a; green - phosphohistone H3.
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present S6B right (3rd row) , S6B right upper , S6B Sp2/+ , S6B right (2nd row) , S6B right lower
Specimen S8 HNF4A WT: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present S8_HNF4A WT
Specimen S8 HNF4A Sp2/+: embryonic day 16.5; Hnf1btm2.1Sce/Hnf1b+ (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Weak S8_HNF4A Sp2/+ Expression strongly downregulated compared to wild type.
Specimen S8 HNF4A Hnf1a-/: embryonic day 16.5; Hnf1atm1.1Ylee/Hnf1atm1.1Ylee (more )
Structure Level Pattern Image Note
TS24: cortical early proximal tubule Present S8_HNF4A Hnf1a-/- Expression not downregulated compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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