Cux1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:294282 Li L, et al., The COMPASS Family Protein ASH2L Mediates Corticogenesis via Transcriptional Regulation of Wnt Signaling. Cell Rep. 2019 Jul 16;28(3):698-711.e5
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7855681
Gene symbol:	Cux1
Gene name:	cut-like homeobox 1

Antibody:

Results

Specimen 1E Cux1 Control: postnatal day 3; Tg(Dach1-cre)1Krs/0 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neocortex	pyramidal neuron	Present	Regionally restricted	1E Cux1 Control	Expression in upper layer projection neurons.

1E Cux1 cKO ©

Specimen 1E Cux1 cKO: postnatal day 3; Ash2l^tm1.1Bcgen/Ash2l^tm1.1Bcgen, Tg(Dach1-cre)1Krs/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Weak	Regionally restricted	1E Cux1 cKO	Signficant reduction in number of expressing cells compared to control.

1F Control ©

Specimen 1F Cux1 Control: postnatal day 3; Tg(Dach1-cre)1Krs/0 (more

)
Note: Triple labeled: gray - Cux1; green - Bcl11b (assay MGI:7855683); red - Tle4 (assay MGI:7855684).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neocortex	pyramidal neuron	Present	Regionally restricted	1F Control	Expression in upper layer projection neurons.

1F cKO ©

1F cKO inset ©

Specimen 1F Cux1 cKO: postnatal day 3; Ash2l^tm1.1Bcgen/Ash2l^tm1.1Bcgen, Tg(Dach1-cre)1Krs/0 (more

)
Note: Conditional mutant. Triple labeled: gray - Cux1; green - Bcl11b (assay MGI:7855683); red - Tle4 (assay MGI:7855684).

Structure	Level	Pattern	Image	Note
TS27: neocortex	Weak	Regionally restricted	1F cKO inset , 1F cKO	Expressing cells misplaced in deep cortical layers and underlying white matter.

Specimen S1H Cux1 Control: postnatal day 7; Tg(Dach1-cre)1Krs/0 (more

)

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Regionally restricted	S1H Cux1 Control	Expression in upper layer projection neurons.

S1H Cux1 cKO ©

Specimen S1H Cux1 cKO: postnatal day 7; Ash2l^tm1.1Bcgen/Ash2l^tm1.1Bcgen, Tg(Dach1-cre)1Krs/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Weak	Regionally restricted	S1H Cux1 cKO	Authors report decreased upper layer marker expression.

Specimen 2B Cux1 Control: postnatal day 3; Tg(Dach1-cre)1Krs/0 (more

)
Note: Double labeled: green - Cux1; red - Edu.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present		2B Cux1 Control

2B Cux1 cKO ©

Specimen 2B Cux1 cKO: postnatal day 3; Ash2l^tm1.1Bcgen/Ash2l^tm1.1Bcgen, Tg(Dach1-cre)1Krs/0 (more

)
Note: Conditional mutant. Double labeled: green - Cux1; red - Edu.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present		2B Cux1 cKO inset , 2B Cux1 cKO

Specimen 2C Cux1 Control: postnatal day 3; Tg(Dach1-cre)1Krs/0 (more

)
Note: Double labeled: green - Cux1; red - Edu.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present		2C Cux1 Control

2C Cux1 cKO ©

Specimen 2C Cux1 cKO: postnatal day 3; Ash2l^tm1.1Bcgen/Ash2l^tm1.1Bcgen, Tg(Dach1-cre)1Krs/0 (more

)
Note: Conditional mutant. Double labeled: green - Cux1; red - Edu.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present		2C Cux1 cKO

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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