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Gene Expression Data
Assay Details
Assay
Reference: J:174095 Cai Z, et al., Loss of shp2-mediated mitogen-activated protein kinase signaling in muller glial cells results in retinal degeneration. Mol Cell Biol. 2011 Jul;31(14):2973-83
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7788479
Gene symbol: Mapk1
Gene name: mitogen-activated protein kinase 1
Antibody: anti-phospho-ERK1/2 (D13.14.4E; #4370)
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; both family members have been annotated. A tyramide-enhanced immunofluorescence technique was used.
Results
Specimen 3E/F/H WT: postnatal day 21 (more )
Note: Double labeled: red - Mapk1 and Mapk3; green - Glul (assay MGI:7788465).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Weak 3F WT , 3E WT , 3H WT Coexpressed with glutamine synthetase in the cell bodies.
TS28: retina ganglion cell layer Weak 3E WT
Specimen 3E/F/H cKO: postnatal day 21; Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Double labeled: red - Mapk1 and Mapk3; green - Glul (assay MGI:7788465).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Absent 3F cKO , 3E cKO , 3H cKO
TS28: retina ganglion cell layer Weak 3E cKO
Specimen 6K: postnatal day 21 (more )
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Weak 6K
Specimen 6L: postnatal day 21; Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Absent 6L
Specimen 6M: postnatal day 21; Krastm4Tyj/Krastm4Tyj, Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Weak 6M Expression was restored in the Muller glial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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