Smo Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:353362 Walker MF, et al., GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade. PLoS Biol. 2024 Aug;22(8):e3002685
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7734742
Gene symbol:	Smo
Gene name:	smoothened, frizzled class receptor

Antibody:	anti-pSMO (7TM0239A)
Detection system:	Secondary antibody coupled to Alexa Fluor 568
Assay notes:	The antibody used in this assay recognizes the phosphorylated form of the protein.

Results

Specimen 7A Smo^bnb: embryonic day 9.5; Smo^bnb/Smo^bnb (more

)
Note: Ventral spinal cord. Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		7A pSMO Smo^bnb , 7A Merge Smo^bnb

Specimen 7A Smo^cbb: embryonic day 9.5; Smo^cbb/Smo^cbb (more

)
Note: Ventral spinal cord. Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A pSMO Smo^cbb , 7A Merge Smo^cbb	The percentage of the neural tube stained in the hypomorph is less than in wild type.

7A pSMO Smo^WT ©

7A Smo^WT ©

Specimen 7A Smo^WT: embryonic day 9.5 (more

)
Note: Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A Merge Smo^WT , 7A pSMO Smo^WT , 7A Smo^WT	The cilia in the ventral neural tube, where Shh levels are the highest, are prominently stained; those in the dorsal neural tube, where levels of Shh are low, are not.

Specimen 7A Ptch1^-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Ventral spinal cord. Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A Merge Ptch1^-/- , 7A pSMO Ptch1^-/-	The percentage of the neural tube stained in the mutant is greater than in wild type.

S9A Smo^WT ©

Specimen S9A Smo^WT: embryonic day 9.5 (more

)
Note: Dorsal spinal cord. Triple labeled: green - Smo; red - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		S9A Smo^WT , S9A pSMO Smo^WT , S9A Merge Smo^WT	The cilia in the ventral neural tube, where Shh levels are the highest, are prominently stained; those in the dorsal neural tube, where levels of Shh are low, are not.

Specimen S9A Smo^bnb: embryonic day 9.5; Smo^bnb/Smo^bnb (more

)
Note: Dorsal spinal cord. Triple labeled: green - Smo; red - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		S9A Merge Smo^bnb , S9A pSMO Smo^bnb

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 01/06/2026 MGI 6.24