Krt5 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279388 Weiss AC, et al., Delayed onset of smooth muscle cell differentiation leads to hydroureter formation in mice with conditional loss of the zinc finger transcription factor gene Gata2 in the ureteric mesenchyme. J Pathol. 2019 Aug;248(4):452-463
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7718874
Gene symbol:	Krt5
Gene name:	keratin 5

Antibody:

Anti-Krt5

Results

Specimen 2Y: embryonic day 18.5; Gata2^tm1Sac/? (more

)
Note: Littermates without the Tbx18^cre were used as control. Double labeled: green - Krt5; purple - Trp63 (assay MGI:7718871).

Structure	Cell Type	Level	Image	Note
TS26: urothelium of ureter	basal cell of urothelium	Present	2Y	Expression of KRT5, deltaNP63, and UPK1B combinatorially mark basal cells (KRT5+deltaNP63+UPK1B-), intermediate cells (KRT5-deltaNP63+UPK1B+), and superficial cells (KRT5-deltaNP63-UPK1B+) in the urothelium.
TS26: urothelium of ureter	intermediate cell of urothelium	Absent	2Y	Expression of KRT5, deltaNP63, and UPK1B combinatorially mark basal cells (KRT5+deltaNP63+UPK1B-), intermediate cells (KRT5-deltaNP63+UPK1B+), and superficial cells (KRT5-deltaNP63-UPK1B+) in the urothelium.
TS26: urothelium of ureter	umbrella cell of urothelium	Absent	2Y	Expression of KRT5, deltaNP63, and UPK1B combinatorially mark basal cells (KRT5+deltaNP63+UPK1B-), intermediate cells (KRT5-deltaNP63+UPK1B+), and superficial cells (KRT5-deltaNP63-UPK1B+) in the urothelium.

Specimen 2Z: embryonic day 18.5; Gata2^tm1Sac/Gata2^tm1Sac, Tbx18^tm4(cre)Akis/Tbx18⁺ (more

)
Note: Conditional mutant. Double labeled: green - Krt5; purple - Trp63 (assay MGI:7718871).

Structure	Cell Type	Level	Image	Note
TS26: urothelium of ureter	basal cell of urothelium	Present	2Z	Expression was not affected in Gata2cKO.
TS26: urothelium of ureter	intermediate cell of urothelium	Absent	2Z	Expression was not affected in Gata2cKO.
TS26: urothelium of ureter	umbrella cell of urothelium	Absent	2Z	Expression was not affected in Gata2cKO.

Specimen S4M: embryonic day 18.5; Gata2^tm1Sac/? (more

)
Note: Littermates without the Tbx18^cre were used as control. Double labeled: green - Krt5; purple - Trp63 (assay MGI:7718871).

Structure	Cell Type	Level	Image	Note
TS26: urothelium of ureter	basal cell of urothelium	Present	S4M	Expression of KRT5, deltaNP63, and UPK1B combinatorially mark basal cells (KRT5+deltaNP63+UPK1B-), intermediate cells (KRT5-deltaNP63+UPK1B+), and superficial cells (KRT5-deltaNP63-UPK1B+) in the urothelium.
TS26: urothelium of ureter	intermediate cell of urothelium	Absent	S4M	Expression of KRT5, deltaNP63, and UPK1B combinatorially mark basal cells (KRT5+deltaNP63+UPK1B-), intermediate cells (KRT5-deltaNP63+UPK1B+), and superficial cells (KRT5-deltaNP63-UPK1B+) in the urothelium.
TS26: urothelium of ureter	umbrella cell of urothelium	Absent	S4M	Expression of KRT5, deltaNP63, and UPK1B combinatorially mark basal cells (KRT5+deltaNP63+UPK1B-), intermediate cells (KRT5-deltaNP63+UPK1B+), and superficial cells (KRT5-deltaNP63-UPK1B+) in the urothelium.

Specimen S4N: embryonic day 18.5; Gata2^tm1Sac/Gata2^tm1Sac, Tbx18^tm4(cre)Akis/Tbx18⁺ (more

)
Note: Conditional mutant. Double labeled: green - Krt5; purple - Trp63 (assay MGI:7718871).

Structure	Cell Type	Level	Image	Note
TS26: urothelium of ureter	basal cell of urothelium	Present	S4N	Expression was not affected in Gata2cKO.
TS26: urothelium of ureter	intermediate cell of urothelium	Absent	S4N	Expression was not affected in Gata2cKO.
TS26: urothelium of ureter	umbrella cell of urothelium	Absent	S4N	Expression was not affected in Gata2cKO.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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