Sox2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:350482 Chander A, et al., Loss of KANSL3 leads to defective inner cell mass and early embryonic lethality. Mol Reprod Dev. 2024 May;91(5):e23760
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7665321
Gene symbol:	Sox2
Gene name:	SRY (sex determining region Y)-box 2

Antibody:	anti-Sox2 (AF2018)
Detection system:	Alexa Fluor

Results

3a CONTROL MERGED ©

3a CONTROL SOX2 ©

Specimen 3a CONTROL: embryonic day 3.5 (more

)
Note: Double labeled: red - Sox2; white - Cdx2 (assay MGI:7665322).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		3a CONTROL SOX2 , 3a CONTROL MERGED

3a MUT MERGED ©

3a MUT SOX2 ©

Specimen 3a MUT: embryonic day 3.5; Kansl3^em1(IMPC)J/Kansl3^em1(IMPC)J (more

)
Note: Double labeled: red - Sox2; white - Cdx2 (assay MGI:7665322).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Weak		3a MUT MERGED , 3a MUT SOX2	Intensity of expression and number of expressing cells significantly lower compared to control.

4a CONTROL MERGED ©

4a CONTROL SOX2 ©

Specimen 4a CONTROL: embryonic day 3.5 (more

)
Note: Triple labeled: red - Sox2; white - Cdx2 (assay MGI:7665322); green - Phospho-H3.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		4a CONTROL SOX2 , 4a CONTROL MERGED

4a MUT MERGED ©

4a MUT SOX2 ©

Specimen 4a MUT: embryonic day 3.5; Kansl3^em1(IMPC)J/Kansl3^em1(IMPC)J (more

)
Note: Triple labeled: red - Sox2; white - Cdx2 (assay MGI:7665322); green - Phospho-H3.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		4a MUT SOX2 , 4a MUT MERGED

5a CONTROL MERGED ©

5a CONTROL SOX2 ©

Specimen 5a CONTROL: embryonic day 3.5 (more

)
Note: Triple labeled: red - Sox2; white - Cdx2 (assay MGI:7665322); green - H4k5ac.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5a CONTROL SOX2 , 5a CONTROL MERGED

5a MUT MERGED ©

5a MUT SOX2 ©

Specimen 5a MUT: embryonic day 3.5; Kansl3^em1(IMPC)J/Kansl3^em1(IMPC)J (more

)
Note: Triple labeled: red - Sox2; white - Cdx2 (assay MGI:7665322); green - H4k5ac.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5a MUT MERGED , 5a MUT SOX2

5c CONTROL MERGED ©

5c CONTROL SOX2 ©

Specimen 5c CONTROL: embryonic day 3.5 (more

)
Note: Triple labeled: red - Sox2; white - Cdx2 (assay MGI:7665322); green - H4k8ac.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5c CONTROL SOX2 , 5c CONTROL MERGED

5c MUT SOX2 ©

Specimen 5c MUT: embryonic day 3.5; Kansl3^em1(IMPC)J/Kansl3^em1(IMPC)J (more

)
Note: Triple labeled: red - Sox2; white - Cdx2 (assay MGI:7665322); green - H4k8ac.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5c MUT SOX2 , 5c MUT MERGED

Specimen 5g CONTROL: embryonic day 3.5 (more

)
Note: Double labeled: red - Sox2; white - H4k16ac.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5g CONTROL MERGED , 5g CONTROL SOX2

5g MUT SOX2 ©

Specimen 5g MUT: embryonic day 3.5; Kansl3^em1(IMPC)J/Kansl3^em1(IMPC)J (more

)
Note: Double labeled: red - Sox2; white - H4k16ac.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5g MUT SOX2 , 5g MUT MERGED

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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