Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:331053
Chandrasekaran P, et al., CXCL12 defines lung endothelial heterogeneity and promotes distal vascular growth. Development. 2022 Nov 1;149(21):dev200909
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:7616684
|
|
Gene symbol:
|
Cxcl12
|
|
Gene name:
|
C-X-C motif chemokine ligand 12
|
|
Reporter:
|
RFP
|
|
Antibody:
|
anti-RFP (600-401-379)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 555
|
|
|
Results
|
Specimen
1A:
(close  )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pulmonary artery |
Strong |
Regionally restricted |
1A
|
High levels of expression in pulmonary arteries marking two primary intrapulmonary branches.
|
Specimen
1B:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pulmonary artery |
Present |
Regionally restricted |
1B
|
Expression in secondary branching from primary intrapulmonary arteries, and in small, thin tubular sprouts growing toward lung periphery.
|
Specimen
1C:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung vascular element |
Present |
Regionally restricted |
1C
|
Expression in secondary branching from primary intrapulmonary arteries, and in small, thin tubular sprouts growing toward lung periphery.
|
Specimen
1D:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung vascular element |
Present |
Regionally restricted |
1D
|
Expression in increased secondary branching, along with tertiary and quaternary arterial branches.
|
Specimen
1E:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung vascular element |
Present |
Regionally restricted |
1E
|
Expression in arterial tree undergoing significant growth and futher branching complexity.
|
Specimen
S1A:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Vwf (assay MGI:7616685).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung vascular element |
Present |
Regionally restricted |
S1A
|
HIghly expressed throughout arterial endothelium, with additional expression in mesenchyme.
|
Specimen
S1B:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Vwf (assay MGI:7616685).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung vascular element |
Strong |
Regionally restricted |
S1B
|
HIghly expressed throughout arterial endothelium, with additional expression in mesenchyme.
|
Specimen
S1C:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
|
Specimen
S1D:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
|
Specimen
S1E:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
|
Specimen
S1F:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
|
Specimen
S1G:
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more  )
Note: Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
Specimen
S1G:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
|
Specimen
S1H:
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Note: Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
Specimen
S1H:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
|
Specimen
S1I:
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Note: Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
Specimen
S1I:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
|
Specimen
S1J:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; green - Acta2 (assay MGI:7616688); white - Emcn (assay MGI:7616686).
|
Specimen
3F:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pulmonary artery |
Present |
Regionally restricted |
3F
|
Expression in proximal artery branch.
|
| TS26: lung vascular element |
Present |
Regionally restricted |
3F
|
Expression in arterial tree from distal tip of secondary branch to quinary branches.
|
Specimen
3G:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
|
Specimen
3H,K:
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Note: Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
Specimen
3H,K:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
|
Specimen
3I,N:
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Note: Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
Specimen
3I,N:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
|
Specimen
S3A:
embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Note: Double labeled: red - Cxcl12 reporter; white - Gkn3 (assay MGI:7616702).
Specimen
S3A:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Cxcl12 reporter; white - Gkn3 (assay MGI:7616702).
|
Specimen
S3C:
embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Note: Double labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703).
Specimen
S3C:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703).
|
Specimen
S3F:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S3I:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S3L:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
5A:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
5F:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
5J (upper):
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Specimen
5J (upper):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
5J (lower):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5A:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5B:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung vascular element |
Present |
Regionally restricted |
S5B
|
Distally, arteries lack directionality toward periphery.
|
Specimen
S5F (upper):
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Specimen
S5F (upper):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5F (lower):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5G (upper):
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Specimen
S5G (upper):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5G (lower):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5H (upper):
embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12 +
(more )
Specimen
S5H (upper):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S5H (lower):
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
7A:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12+
Tg(Tek-cre)12Flv/0
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
7B:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Mutant Allele(s):
|
Cxcl12tm2.1Sjm/Cxcl12tm1.1Sjm
Tg(Tek-cre)12Flv/0
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Conditional mutant.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung vascular element |
Present |
Regionally restricted |
7B
|
Authors report primary branching defects, and decreased secondary branch numbers.
|
|
Analysis Tools