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Gene Expression Data
Assay Details
Assay
Reference: J:262420 Szenker-Ravi E, et al., RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6. Nature. 2018 May;557(7706):564-569
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7596300
Gene symbol: Vim
Gene name: vimentin
Antibody: Anti-Vimentin (EPR3776; ab92547)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 2i WT (left): embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: lung Present 2i Lgr4/5/6 +/+ Vimentin
Specimen 2i WT inset (left): embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: lung Present 2i Lgr4/5/6 +/+ Vimentin inset
Specimen 2i Triple KO (left): embryonic day 14.5; Lgr4tm1(cre/ERT2)Cle/Lgr4tm1(cre/ERT2)Cle, Lgr5tm1.1(cre/ERT2)Npa/Lgr5tm1.1(cre/ERT2)Npa, Lgr6tm2.1(cre/ERT2)Cle/Lgr6tm2.1(cre/ERT2)Cle (more )
Note: Double labeled: red - Vim; green - Lgr4 (assay MGI:7596302) and Lgr5 reporter (assay MGI:7606801) and Lgr6 reporter (assay MGI:7606802).
Structure Level Pattern Image Note
TS22: lung Present 2i Lgr4/5/6 -/- Vimentin
Specimen 2i Triple KO inset (left): embryonic day 14.5; Lgr4tm1(cre/ERT2)Cle/Lgr4tm1(cre/ERT2)Cle, Lgr5tm1.1(cre/ERT2)Npa/Lgr5tm1.1(cre/ERT2)Npa, Lgr6tm2.1(cre/ERT2)Cle/Lgr6tm2.1(cre/ERT2)Cle (more )
Note: Double labeled: red - Vim; green - Lgr4 (assay MGI:7596302) and Lgr5 reporter (assay MGI:7606801) and Lgr6 reporter (assay MGI:7606802).
Structure Level Pattern Image Note
TS22: lung Present 2i Lgr4/5/6 -/- Vimentin inset
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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