Antibody:	anti-Cer1
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS9: anterior visceral endoderm	Strong		2I Gata6/Dkk1/Cer1 (lower) , 2I Gata6/Dkk1/Cer1 (upper) , 2I Cer1 (upper) , 2I Single section , 2Ib (upper) , 2Ia (lower) , 2I Dkk1/Cer1 (lower) , 2I Z-stack (lower) , 2I Dkk1/Cer1 (upper) , 2Ia (upper) , 2I Cer1 (lower) , 2I DAPI/Dkk1/Cer1 (upper) , 2I DAPI/Dkk1/Cer1 (lower) , 2I Z-stack (upper) , 2Ib (lower)	High expression in late anterior visceral endoderm (L-AVE).
TS9: posterior visceral endoderm	Absent		2I Dkk1/Cer1 (upper) , 2Ia (lower) , 2I DAPI/Dkk1/Cer1 (upper) , 2I Dkk1/Cer1 (lower) , 2I Gata6/Dkk1/Cer1 (lower) , 2Ib (lower) , 2Ib (upper) , 2I Cer1 (lower) , 2Ia (upper) , 2I DAPI/Dkk1/Cer1 (lower) , 2I Gata6/Dkk1/Cer1 (upper) , 2I Cer1 (upper)	Expression low or absent in late distal visceral endoderm (L-DVE) located above L-AVE.

Structure	Level	Pattern	Image	Note
TS9: anterior visceral endoderm	Present	Regionally restricted	3K Cer1 , 3K merge (upper) , 3K inset (upper) , 3K Cer1/Tox3	Expression in L-AVE, colocalized with Tox3 expression.

Structure	Level	Pattern	Image	Note
TS7: anterior visceral endoderm	Present	Regionally restricted	3L Cer1/Tox3 , 3L Cer1 , 3L inset , 3L merge	Expression in L-AVE and early anterior visceral endoderm (E-AVE).
TS7: posterior visceral endoderm	Present	Regionally restricted	3L Cer1 , 3L merge , 3L inset , 3L Cer1/Tox3	Expression in distal visceral endoderm (E-DVE), located in front of E-AVE.

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present	Regionally restricted	4L inset , 4L Cer1 , 4L merge , 4L Cer1/Gpr83	Expression in a population of cells previously identified as distal visceral endoderm, possibly pre-anterior visceral endoderm (pre-AVE).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present	Regionally restricted	S4A inset , S4A merge , S4A Cer1/Gpr83 , S4A Cer1	Expression in a population of cells previously identified as distal visceral endoderm, possibly pre-anterior visceral endoderm (pre-AVE).