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Gene Expression Data
Assay Details
Assay
Reference: J:332652 Zhu Q, et al., Decoding anterior-posterior axis emergence among mouse, monkey, and human embryos. Dev Cell. 2023 Jan 9;58(1):63-79.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7580999
Gene symbol: Dkk1
Gene name: dickkopf WNT signaling pathway inhibitor 1
Antibody: Dkk-1 Antibody (AF1765)
Detection system: Secondary antibody
Results
Specimen 2I: embryonic day 6.5 (more )
Note: Triple labeled: green - Dkk1; red - Cer1 (assay MGI:7581000); blue - Gata6 (assay MGI:7581001).
Structure Level Pattern Image Note
TS9: posterior visceral endoderm Present Regionally restricted 2I Dkk1 (upper) , 2I Dkk1/Cer1 (upper) , 2I Dkk1/Cer1 (lower) , 2I Gata6/Dkk1/Cer1 (upper) , 2I Z-stack (lower) , 2I DAPI/Dkk1/Cer1 (lower) , 2Ia (upper) , 2I Single section , 2Ia (lower) , 2I Dkk1 (lower) , 2Ib (lower) , 2I Gata6/Dkk1/Cer1 (lower) , 2Ib (upper) , 2I Z-stack (upper) Expression in late distal visceral endoderm (L-DVE) located above late anterior visceral endoderm (L-AVE) in anterior or embryos.
TS9: anterior visceral endoderm Absent 2Ia (lower) , 2I DAPI/Dkk1/Cer1 (lower) , 2Ib (lower) , 2I Dkk1/Cer1 (lower) , 2I Gata6/Dkk1/Cer1 (lower) , 2Ia (upper) , 2I Gata6/Dkk1/Cer1 (upper) , 2I Dkk1 (lower) , 2Ib (upper) , 2I Dkk1/Cer1 (upper) , 2I Dkk1 (upper) , 2I DAPI/Dkk1/Cer1 (upper) Expression absent in late anterior visceral endoderm (L-AVE).
Specimen 3K (lower): embryonic day 6.5 (more )
Note: Double labeled: red - Dkk1; green - Tox3 (assay MGI:7581002).
Structure Level Pattern Image Note
TS9: posterior visceral endoderm Present Regionally restricted 3K merge (lower) , 3K inset (lower) , 3K Dkk1/Tox3 , 3K Dkk1 Expression in L-DVE, located above L-AVE.
TS9: anterior visceral endoderm Absent 3K Dkk1 , 3K Dkk1/Tox3 , 3K inset (lower) , 3K merge (lower)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory