Gene Expression Data
Assay Details
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Assay
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Reference:
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J:93057
Schuurmans C, et al., Sequential phases of cortical specification involve Neurogenin-dependent and -independent pathways. EMBO J. 2004 Jul 21;23(14):2892-902
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7578965
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Gene symbol:
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Gad1
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Gene name:
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glutamate decarboxylase 1
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Probe:
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GAD67 cDNA
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Probe preparation:
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Antisense RNA
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Results
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Specimen
1N:
embryonic day 13.5
(more  )
Specimen
1N:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
Regionally restricted |
1N
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Expressed in the subcortical region.
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Specimen
1O:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog2tm2Fgu/Neurog2tm2Fgu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1P:
(close )
Specimen
1Q:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1R/S:
embryonic day 13.5
(more )
Note: Double labeled: blue - Gad1; brown - Slc17a7 (assay MGI:7578966).
Specimen
1R/S:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gad1; brown - Slc17a7 (assay MGI:7578966).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
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1S
,
1R
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For the most part, no co-expression with Slc17a7/VGLUT1.
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Specimen
1T/U:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ
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Mutant Allele(s):
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Neurog1tm1And/Neurog1tm1And
Neurog2tm2Fgu/Neurog2tm2Fgu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gad1; brown - Slc17a7 (assay MGI:7578966).
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Specimen
not shown Ngn2-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog2tm2Fgu/Neurog2tm2Fgu
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical plate |
Present |
Clusters |
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Ectopic expression as large aggregates in lower region was seen, and was excluded from the more recently generated, superficial layer.
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Specimen
not shown Ngn1-/-;Ngn2-/-:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical plate |
Present |
Clusters |
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Ectopic expression as large aggregates in lower region was seen, and was excluded from the more recently generated, superficial layer.
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Specimen
not shown Ngn2-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog2tm2Fgu/Neurog2tm2Fgu
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Clusters |
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Ectopically expressed in small clusters in the germinal zone, rostral marginal zone and flanking the intermediate zone/lower cortical plate border.
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Specimen
not shown Ngn1-/-;Ngn2-/-:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Clusters |
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Ectopically expressed in small clusters in the germinal zone, rostral marginal zone and flanking the intermediate zone/lower cortical plate border.
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Specimen
not shown Pax6-/-:
embryonic day 15.5; Pax6Sey/ Pax6Sey
(more )
Specimen
not shown Pax6-/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
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Ectopic upregulation of expression was seen, compared with the wild-type.
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Specimen
not shown Pax6-/-;Tlx-/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Nr2e1tm1Rtyu/Nr2e1tm1Rtyu
Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
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Ectopic upregulation of expression was seen, compared with the wild-type.
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Specimen
not shown Tlx-/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Nr2e1tm1Rtyu/Nr2e1tm1Rtyu
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Clusters |
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Expressed in small ectopic clusters in the germinal zone.
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Specimen
not shown Pax6-/-:
embryonic day 18.5; Pax6Sey/ Pax6Sey
(more )
Specimen
not shown Pax6-/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
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Expressed in a grossly expanded germinal zone, compared with the wild-type.
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Specimen
not shown Pax6-/-;Tlx-/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Nr2e1tm1Rtyu/Nr2e1tm1Rtyu
Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
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Expressed in a grossly expanded germinal zone, compared with the wild-type.
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