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Gene Expression Data
Assay Details
Assay
Reference: J:226892 Hammond VE, et al., Ndfip1 is required for the development of pyramidal neuron dendrites and spines in the neocortex. Cereb Cortex. 2014 Dec;24(12):3289-300
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7571610
Gene symbol: Ndfip1
Gene name: Nedd4 family interacting protein 1
Antibody: anti-Ndfip1 (1G5)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1B: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: cortical plate neuron Present 1B
Specimen 1C: postnatal day 7 (more )
Note: Double labeled: red - Ndfip1; green - Emx1 (assay MGI:7572420).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex pyramidal neuron Present 1C Expressed in all Emx1+ neurons. Expression was observed in the neuronal soma, in which it exhibited a perinuclear distribution with strong expression in apical processes and dendrites.
Specimen 2A inset: postnatal day 30; Ndfip1tm1Sest/Ndfip1tm1Sest (more )
Note: Double labeled: red - Ndfip1; green - GABA.
Structure Cell Type Level Pattern Image Note
TS28: neocortex GABAergic neuron Present 2A inset Co-expressed with GABA in some of the interneurons.
TS28: neocortex pyramidal neuron Present Scattered 2A inset Expressed in GABA- pyramidal neurons.
Specimen 2C inset: postnatal day 30; Emx1tm1.1(cre)Ito/Emx1+, Ndfip1tm1Sest/Ndfip1tm1Sest (more )
Note: Conditional mutant. Double labeled: red - Ndfip1; green - GABA.
Structure Cell Type Level Pattern Image Note
TS28: neocortex pyramidal neuron Absent 2C inset No expression in pyramidal neurons.
TS28: neocortex GABAergic interneuron Present Scattered 2C inset Expression in GABA+ interneurons remained.
Specimen S1A/C: postnatal day 1 (more )
Note: Double labeled: red - Ndfip1; green - Pten (assay MGI:7572410).
Structure Cell Type Level Pattern Image Note
TS27: cortical plate neuron Strong S1A , S1C Co-expressed with Pten.
Specimen S1D/F: postnatal day 1 (more )
Note: Double labeled: red - Ndfip1; green - Nedd4l (assay MGI:7572411).
Structure Cell Type Level Pattern Image Note
TS27: cortical plate neuron Strong S1D , S1F Co-expressed with Nedd4l.
Specimen S1G/I: postnatal day 1 (more )
Note: Double labeled: red - Ndfip1; green - Akt1 (assay MGI:7572412).
Structure Cell Type Level Pattern Image Note
TS27: cortical plate neuron Strong S1G , S1I Co-expressed with phos-Akt1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory