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Gene Expression Data
Assay Details
Assay
Reference: J:284860 Okashita N, et al., TET2 catalyzes active DNA demethylation of the Sry promoter and enhances its expression. Sci Rep. 2019 Sep 17;9(1):13462
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7563886
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: Anti-Sox9 (AB5535)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4b Tet2delta/+: embryonic day 13.5; Tet2em1Mtac/Tet2+ (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4b XY

Specimen 4b Tet2delta/delta XY: embryonic day 13.5; Tet2em1Mtac/Tet2em1Mtac (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4b XY middle

Specimen 4b Tet2delta/delta XX: embryonic day 13.5; Tet2em1Mtac/Tet2em1Mtac (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Level Pattern Image Note
TS21: mesenchymal stroma of ovary Absent 4b XX

Specimen 4d Jmjd1adelta/+;Tet2delta/delta XY: embryonic day 13.5; Kdm3atm1.1Jsak/Kdm3a+, Tet2em1Mtac/Tet2em1Mtac (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4d XY Jmjd1adelta/+;Tet2delta/delta

Specimen 4d Jmjd1adelta/delta;Tet2+/+: embryonic day 13.5; Kdm3atm1.1Jsak/Kdm3atm1.1Jsak (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4d XY Jmjd1adelta/delta;Tet2+/+ This mutant has an ovotestes composed of testicular cells and ovarian cells. The ratio of FOXL2-positive cells to SOX9-positive cells was increased in Tet2/Kdm3a-double deficient gonads as compared to Kdm3a-deficient gonads.

Specimen 4d Jmjd1adelta/delta;Tet2delta/delta: embryonic day 13.5; Kdm3atm1.1Jsak/Kdm3atm1.1Jsak, Tet2em1Mtac/Tet2em1Mtac (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4d XY Jmjd1adelta/delta;Tet2delta/delta This mutant has an ovotestes composed of testicular cells and ovarian cells. The ratio of FOXL2-positive cells to SOX9-positive cells was increased in Tet2/Kdm3a-double deficient gonads as compared to Kdm3a-deficient gonads.

Specimen 4d Jmjd1adelta/+;Tet2delta/delta XX: embryonic day 13.5; Kdm3atm1.1Jsak/Kdm3a+, Tet2em1Mtac/Tet2em1Mtac (more )
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7563889).
Structure Level Pattern Image Note
TS21: ovary Absent 4d XX Jmjd1adelta/+;Tet2delta/delta


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory