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Gene Expression Data
Assay Details
Assay
Reference: J:221086 Patra D, et al., Cartilage-specific ablation of site-1 protease in mice results in the endoplasmic reticulum entrapment of type IIb procollagen and down-regulation of cholesterol and lipid homeostasis. PLoS One. 2014;9(8):e105674
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7537305
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Antibody: anti-Col IIA (IIA)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2H/I: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7537302).
Structure Level Pattern Image Note
TS24: femur Present Regionally restricted 2I , 2H Expression only in cartilage matrix outside of cells, forming an organized lattice.
Specimen 2K/L: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7537302).
Structure Level Pattern Image Note
TS24: femur Present Regionally restricted 2L , 2K Expression in an organized lattice in cartilage matrix.
Specimen 9C: embryonic day 15.5 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7537302).
Structure Level Pattern Image Note
TS23: humerus Present 9C
Specimen 9D: embryonic day 15.5; Scd1tm1Ntam/Scd1tm1Ntam (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7537302).
Structure Level Pattern Image Note
TS23: humerus Present 9D
Specimen 9E: embryonic day 15.5 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7537302).
Structure Level Pattern Image Note
TS23: humerus Present 9E
Specimen 9F: embryonic day 15.5; Scd1tm1Ntam/Scd1tm1Ntam (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7537302).
Structure Level Pattern Image Note
TS23: humerus Present 9F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory