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Gene Expression Data
Assay Details
Assay
Reference: J:221086 Patra D, et al., Cartilage-specific ablation of site-1 protease in mice results in the endoplasmic reticulum entrapment of type IIb procollagen and down-regulation of cholesterol and lipid homeostasis. PLoS One. 2014;9(8):e105674
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7537302
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Antibody: anti-Col II THD (IIF)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1B/C: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh (more )
Note: Double labeled: red - Col2a1; green - Canx (assay MGI:7537301).
Structure Level Pattern Image Note
TS24: femur Strong Regionally restricted 1C , 1B Strong expression in matrix of cartilage surrounding chondrocytes. Expression is distinct from that of Canx.
Specimen 1E/F: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: red - Col2a1; green - Canx (assay MGI:7537301).
Structure Level Pattern Image Note
TS24: femur Present Regionally restricted 1F , 1E Expression primarily inside chondrocytes, colocalized with Canx expression, with drastic drop in cartilage matrix expression.
Specimen 1H/I: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh (more )
Note: Double labeled: red - Col2a1; green - Xbp1 (assay MGI:7537306).
Structure Level Pattern Image Note
TS24: femur Present 1H , 1I
Specimen 1K/L: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: red - Col2a1; green - Xbp1 (assay MGI:7537306).
Structure Cell Type Level Pattern Image Note
TS24: femur chondrocyte Present Regionally restricted 1L , 1K Expression co-localized with Xbp1 in chondrocytes.
Specimen 2C/E: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537304).
Structure Level Pattern Image Note
TS24: femur Present Regionally restricted 2C , 2E
Specimen 2D/F: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537304).
Structure Cell Type Level Pattern Image Note
TS24: femur chondrocyte Present Regionally restricted 2F , 2D Expression primarily inside chondrocytes, ovelapping with intracellular expression of pro-Col IIB. Hardly any expression in cartilage matrix.
Specimen 2G/I: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537305).
Structure Level Pattern Image Note
TS24: femur Present 2G , 2I
Specimen 2J/L: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537305).
Structure Level Pattern Image Note
TS24: femur Present Regionally restricted 2L , 2J Expression does not show as an organized lattice.
Specimen 3G: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh (more )
Note: Double labeled: red - Col2a1; green - Comp (assay MGI:7537307).
Structure Level Pattern Image Note
TS24: femur Present Regionally restricted 3G Expression overlapping with that of Comp in matrix. Very little expression detected intracellularly.
Specimen 3H: embryonic day 16.5; Mbtps1tm1Jdh/Mbtps1tm1Jdh, Tg(Col2a1-cre)1Bhr/0 (more )
Note: Conditional mutant. Double labeled: red - Col2a1; green - Comp (assay MGI:7537307).
Structure Cell Type Level Pattern Image Note
TS24: femur chondrocyte Present Regionally restricted 3H Most expression detected intracellularly, in a distinct domain compared to Comp expression.
Specimen 9C: embryonic day 15.5 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537305).
Structure Level Pattern Image Note
TS23: humerus Present 9C
Specimen 9D: embryonic day 15.5; Scd1tm1Ntam/Scd1tm1Ntam (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537305).
Structure Level Pattern Image Note
TS23: humerus Present 9D
Specimen 9E: embryonic day 15.5 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537305).
Structure Level Pattern Image Note
TS23: humerus Present 9E
Specimen 9F: embryonic day 15.5; Scd1tm1Ntam/Scd1tm1Ntam (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7537305).
Structure Level Pattern Image Note
TS23: humerus Present 9F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory