Antibody:	Anti-Sirt2 S8447
Detection system:	Secondary antibody coupled to other - see notes
Assay notes:	Secondary antibody was conjugated with either Cy3 or Alexa Fluor 568.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebral cortex		Present	Regionally restricted	3A left , 3A right	Expression was detected in fiber tracts.
TS28: cerebral cortex	cerebral cortex neuron	Present	Widespread	3A left , 3A right	Expression was detected in neurons (co-labeled with Rbfox3) throughout the cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		3B left , 3B right
TS28: cerebellum molecular layer	cerebellar neuron	Present		3B right , 3B left
TS28: cerebellum molecular layer		Present	Regionally restricted	3B left , 3B right	Expression was detected in fiber tracts.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	oligodendrocyte	Present	Regionally restricted	3C left , 3C right	Expression overlapped with the oligodendroglial marker CNP in fiber tracts, but not in the oligodendroglial cell bodies.
TS28: cerebellum molecular layer	cerebellar neuron	Present		3C left , 3C right
TS28: cerebellum molecular layer		Present	Regionally restricted	3C left , 3C right	Expression was detected in fiber tracts.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	astrocyte	Absent		3D left , 3D right	Staining did not coincide with that of the astrocyte marker Gfap.
TS28: cerebellum molecular layer	cerebellar neuron	Present		3D left , 3D right
TS28: cerebellum molecular layer		Present	Regionally restricted	3D left , 3D right	Expression was detected in fiber tracts.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	S5a right , S5a left	Expression was detected in the fiber tracts. No overlap with CNP was observed in oligodendroglial cell bodies.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain	astrocyte	Absent		S5b left , S5b right	No overlap of staining was observed with Gfap in astrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus striatum	astrocyte	Absent		S5c left , S5c right	No overlap of staining was observed with Gfap in astrocytes.