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Gene Expression Data
Assay Details
Assay
Reference: J:256987 Shi B, et al., MORC2B is essential for meiotic progression and fertility. PLoS Genet. 2018 Jan;14(1):e1007175
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7509248
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phosphoH2AX (#05-636; JBW301)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5A gH2AX leptotene: postnatal day 25 (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5A leptotene merged Expression on autosomal chromatin, distributed widely throughout nucleus.
Specimen 5A gH2AX zygotene: postnatal day 25 (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5A zygotene merged Expression on autosomal chromatin, distributed widely throughout nucleus.
Specimen 5A gH2AX pachytene: postnatal day 25 (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5A pachytene merged Expression restricted to XY chromatin.
Specimen 5A gH2AX diplotene: postnatal day 25 (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5A diplotene merged Expression restricted to XY chromatin.
Specimen 5B gH2AX leptotene: postnatal day 25; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Strong 5B leptotene merged Strong expression in leptotene and zygotene spermatocytes.
Specimen 5B gH2AX zygotene-like: postnatal day 25; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Strong 5B zygotene-like merged Strong expression in leptotene and zygotene spermatocytes.
Specimen 5B gH2AX pachytene-like: postnatal day 25; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: green - H2ax; red - Sycp2 (assay MGI:7509245).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 5B pachytene-like merged Express throughout nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory