Antibody:	anti-Prox1 (AF2727)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present	Regionally restricted	2B	Expression was detected in dermal lymphatic vessels in the dorsal skin.

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present	Regionally restricted	2E	Expression was detected in dermal lymphatic vessels, which were wider in the mutant than the control.

Structure	Level	Pattern	Image	Note
TS26: lymphatic vessel	Present	Regionally restricted	2G	Expression was detected in mesenteric lymphatic vessels. Higher staining was noted in the valves.

Structure	Level	Pattern	Image	Note
TS26: lymphatic vessel	Present	Regionally restricted	2J	Expression was detected in mesenteric lymphatic vessels. A reduced number of higher staining valves was observed in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lymphatic vessel	endothelial cell of lymphatic vessel	Present		4A

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lymphatic vessel	endothelial cell of lymphatic vessel	Present		4B

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lymphatic vessel	endothelial cell of lymphatic vessel	Present		4E

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lymphatic vessel	endothelial cell of lymphatic vessel	Present		4F

Structure	Cell Type	Level	Pattern	Image	Note
TS19: cardinal vein	endothelial cell	Present	Regionally restricted	4L	High expression was detected in lymphatic endothelial progenitor cells upon their exit from the cardinal veins at E11.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: cardinal vein	endothelial cell	Present	Regionally restricted	4M	High expression was detected in lymphatic endothelial progenitor cells upon their exit from the cardinal veins at E11.5. Less ellipticity of the nucleus was noted in the mutant compared with control.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: lymphatic vessel	endothelial cell	Present		4O	High expression was detected in mesenteric valve endothelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: lymphatic vessel	endothelial cell	Present	Regionally restricted	4P	High expression was detected in mesenteric valve endothelial cells. Less ellipticity of the nucleus was noted in the mutant compared with control.

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present	Regionally restricted	S3C	Expression was detected in dermal lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: jugular lymph sac	Present		S3F
TS22: jugular vein	Present		S3F