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Gene Expression Data
Assay Details
Assay
Reference: J:334839 Chuang CK, et al., The Role of SCL Isoforms in Embryonic Hematopoiesis. Int J Mol Sci. 2023 Mar 29;24(7)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466310
Gene symbol: Cd34
Gene name: CD34 antigen
Antibody: anti-CD34 (sc-74499)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2A CD34: embryonic day 10.0 (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Level Pattern Image Note
TS16: yolk sac Present Regionally restricted 2A Expression in hemogenic endothelial cells (HECs).
TS16: dorsal aorta endothelium Present Regionally restricted 2A Expression in thin line on ventral side of aorta.
Specimen 2B CD34: embryonic day 10.0 (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Level Pattern Image Note
TS16: yolk sac Present Regionally restricted 2B CD31/CD34 , 2B CD31/CD34/DiD , 2B CD34 Expression in rectangular and round shaped cells within defined tubular structures in yolk sac.
Specimen 4A CD34 (upper): embryonic day 9.5 (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4A CD34 (upper) , 4A Merge (upper) Expression in dispersed cells in vessel cavities
Specimen 4B CD34 (upper): embryonic day 9.5 (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4B Merge (upper) , 4B CD34 (upper) Expression in dispersed cells in vessel cavities
Specimen 4C CD34 (upper): embryonic day 9.5 (more )
Note: Double labeled: red - Cd34; green - Ptprc (CD45; assay MGI:7466321).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4C CD34 (upper) , 4C Merge (upper) Expression in dispersed cells in vessel cavities
Specimen 5A CD34 (upper): embryonic day 10.5 (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5A CD 34 (upper) , 5A Merge (upper) Co-expression with Cd31 emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5A Merge (upper) , 5A CD 34 (upper) Co-expression with Cd31 in few cells in other lumens.
Specimen 5B CD34 (upper): embryonic day 10.5; Tal1em1Ckc/Tal1+ (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5B CD34 (upper) , 5B Merge (upper) Co-expression with Cd13 emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5B CD34 (upper) , 5B Merge (upper) Co-expression with Cd31 in few cells in other lumens.
Specimen 5C CD34 (upper): embryonic day 10.5; Tal1em1Ckc/Tal1em1Ckc (more )
Note: Double labeled: red - Cd34; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Absent 5C CD34 (upper) , 5C Merge (upper) No expression in endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5C Merge (upper) , 5C CD34 (upper) Co-expression with Cd31 in a few cells in other cavities beyond aorta.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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