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Gene Expression Data
Assay Details
Assay
Reference: J:332999 Kraunsoe S, et al., Requirement for STAT3 and its target, TFCP2L1, in self-renewal of naive pluripotent stem cells in vivo and in vitro. Biol Open. 2023 Jan 1;12(1)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7439561
Gene symbol: Stat3
Gene name: signal transducer and activator of transcription 3
Antibody: anti-phospho-STAT3 (D3A7) (#9145)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Assay notes: The antibody used recognizes the phosphorylated form of the protein. Permeabilisation was performed in methanol prior to staining.
Results
Specimen 1 E3.5: embryonic day 3.5 (more )
Note: Triple labeled: blue - Stat3; green - Tfcp2l1; assay MGI:7439565); magenta - Nanog (assay MGI:7439560).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1B E3.5 pSTAT3(Y705) , 1B E3.5 Merge
Specimen 1 E4.5: embryonic day 4.5 (more )
Note: Triple labeled: blue - Stat3; green - Tfcp2l1; assay MGI:7439565); magenta - Nanog (assay MGI:7439560).
Structure Level Pattern Image Note
TS6: epiblast Present 1B E4.5 pSTAT3(Y705) , 1B E4.5 Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory