Nanog Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:332999 Kraunsoe S, et al., Requirement for STAT3 and its target, TFCP2L1, in self-renewal of naive pluripotent stem cells in vivo and in vitro. Biol Open. 2023 Jan 1;12(1)
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7439560
Gene symbol:	Nanog
Gene name:	Nanog homeobox

Antibody:	anti-Nanog (eBioMLC-51, 14-5761-80)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen 1 E3.5: embryonic day 3.5 (more

)
Note: Triple labeled: magenta - Nanog; green - Tfcp2l1; assay MGI:7439565); blue - Stat3 (assay MGI:7439561).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1B E3.5 Merge , 1B E3.5 NANOG	Expression is in the epiblast.

Specimen 1 E4.5: embryonic day 4.5 (more

)
Note: Triple labeled: magenta - Nanog; green - Tfcp2l1; assay MGI:7439565); blue - Stat3 (assay MGI:7439561).

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		1B E4.5 NANOG , 1B E4.5 Merge

2B ©

Specimen 2B: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		2B	Expression is in the epiblast.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24