Antibody:	anti-YAP (#4912)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: optic cup outer layer	Moderate		1C , 1A	Future pigmented retinal epithelium. The hinge region between neural retina and retinal pigment epithelium, presumptive ciliary body/iris primordium and retinal pigment epithelium showed moderately intense expression.
TS17: diencephalon	Present		1C , 1A
TS17: eye surface ectoderm	Present		1C , 1A
TS17: neural retinal epithelium	Present		1C , 1A
TS17: optic stalk	Present		1C , 1A
TS17: eye mesenchyme	Present	Regionally restricted	1A , 1C	Expressed in periocular mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	1G1 , 1G	Expressed at the apical and basal surfaces and in the cytoplasm of the epithelial cells. Weak expression in the nuclei of a subset of cells in the outer neuroblastic layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: pigmented retinal epithelium	retinal pigment epithelial cell	Present		2D , 2F	Nuclear colocalization with Sox9 was seen.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		3D

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	3E	Yap1-deficient patch was seen.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5E2 , 5E1 , 5E	The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	5F , 5F1 , 5F2	The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression. There was partial fusion when the duplicated retina abutted the original retina.
TS23: pigmented retinal epithelium	Present	Regionally restricted	5F , 5F1 , 5F2	The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression. There was partial fusion when the duplicated retina abutted the original retina.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		6C

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	6D	Yap1-deficient patches were seen.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present		6E , 6F

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	6H , 6I	Yap1-deficient patches were seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: neural retina	retinal progenitor cell	Present	Regionally restricted	S2A , S2D	Most BrdU+ cells overlapped Sox9+Yap+ cells in the outer neuroblastic layer.
TS23: pigmented retinal epithelium	retinal pigment epithelial cell	Present		S2A , S2D	Nuclear colocalization with Sox9 was seen.

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present	Regionally restricted	S3A	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present	Regionally restricted	S3B	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S3D	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S3E	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S3F	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	S3H	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	S3I	Retinal patches in which Yap1 proteins were deficient mixed with patches in which they were preserved.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		S3J

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S3K	Yap1 devoid retinal patches were more frequent in central than peripheral retina.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S3L	Yap1 devoid retinal patches were more frequent in central than peripheral retina.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S4A	Expressed at the apical and basal surfaces and weak expression at the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	S4B	Yap1 devoid retinal patches were seen.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	S4C' , S4C	Expressed at the apical and basal surfaces and the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present		S4D' , S4D	Yap1 devoid retinal patches were seen.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	S4E' , S4E	Expressed at the apical and basal surfaces and the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	S4F' , S4F	Yap1 devoid retinal patches were seen.