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Gene Expression Data
Assay Details
Assay
Reference: J:333036 Zou J, et al., Neddylation is required for perinatal cardiac development through stimulation of metabolic maturation. Cell Rep. 2023 Jan 31;42(1):112018
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7434414
Gene symbol: Hif1a
Gene name: hypoxia inducible factor 1, alpha subunit
Antibody: anti-HIF1a (AF1935)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S7A E9.5: embryonic day 9.5 (more )
Note: Double labeled: green - Hif1a; red - cTnT (Tnnt2 assay MGI:7434416).
Structure Level Pattern Image Note
TS15: cardiac muscle tissue Present S7A E9.5 (merged) , S7A E9.5 HIF1a Abundant expression in mouse heart.
Specimen S7A E11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Hif1a; red - cTnT (Tnnt2 assay MGI:7434416).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Present S7A E11.5 (merged) , S7A E11.5 HIF1a
Specimen S7A E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Hif1a; red - cTnT (Tnnt2 assay MGI:7434416).
Structure Level Pattern Image Note
TS22: cardiac muscle tissue Weak S7A E14.5 (merged) , S7A E14.5 HIF1a Expression downregulated at E14.5.
Specimen 6E CTL: embryonic day 14.5 (more )
Note: Triple labeled: white - Hif1a; red - tdTomato (mT) fluorescence; green - EGFP (mG) fluorescence.
Structure Cell Type Level Pattern Image Note
TS22: cardiac muscle tissue cardiac muscle cell Present Regionally restricted 6E CTL HIF1a+DAPI , 6E CTL HIF1a , 6E CTL Enlarged , 6E CTL Merged Expression in cytosol of cardiomycytes.
Specimen 6E CKO: embryonic day 14.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Nae1tm1c(EUCOMM)Wtsi/Nae1tm1c(EUCOMM)Wtsi, Tg(Myh6-cre)2182Mds/0 (more )
Note: Conditional mutant. Triple labeled: white - Hif1a; red - tdTomato (mT) fluorescence; green - EGFP (mG) fluorescence.
Structure Cell Type Level Pattern Image Note
TS22: cardiac muscle tissue cardiac muscle cell Present Regionally restricted 6E CKO HIF1a , 6E CKO HIF1a+DAPI , 6E CKO Enlarged , 6E CKO Merged Expression in nucleus of cardiomycytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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