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Gene Expression Data
Assay Details
Assay
Reference: J:333074 Ito N, et al., Slc12a8 in the lateral hypothalamus maintains energy metabolism and skeletal muscle functions during aging. Cell Rep. 2022 Jul 26;40(4):111131
Assay type: RNA in situ
MGI Accession ID: MGI:7433136
Gene symbol: Slc12a8
Gene name: solute carrier family 12 (potassium/chloride transporters), member 8
Probe: Mm-Slc12a8 probe1
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 1C: postnatal (more )
Note: Triple labeled: yellow - Slc12a8; green - Slc32a1 (assay MGI:7433151); red - Slc17a6 (assay MGI:7433152).
Structure Cell Type Level Pattern Image Note
TS28: lateral hypothalamic area neuron Present Regionally restricted 1C 20% of the expressing cells are GABAergic and 10% are glutamatergic.
Specimen 1E upper: postnatal (more )
Note: Double labeled: yellow - Slc12a8; green - Hcrt (assay MGI:7433153).
Structure Cell Type Level Pattern Image Note
TS28: lateral hypothalamic area neuron Present Regionally restricted 1E Slc12a8 upper , 1E Merge upper Only 4% of the expressing cells are positive for orexin.
Specimen 1E lower: postnatal (more )
Note: Double labeled: yellow - Slc12a8; green - Pmch (assay MGI:7433154).
Structure Cell Type Level Pattern Image Note
TS28: lateral hypothalamic area neuron Present Regionally restricted 1E Merge lower , 1E Slc12a8 lower Only 3% of the expressing cells are positive for MCH.
Specimen S1: postnatal; Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+, Slc12a8em2(cre/ERT2)Imai/Slc12a8+ (more )
Note: Double labeled: yellow - Slc12a8 RNA; red - Scl12a8 reporter (assay MGI:7433012).
Structure Cell Type Level Pattern Image Note
TS28: lateral hypothalamic area neuron Present Single cells S1C Slc12a8 , S1C Merge Expression of the reporter and endogenous mRNA overlap.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory