L1cam Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:322165 Cunningham JG, et al., Early construction of the thalamocortical axon pathway requires c-Jun N-terminal kinase signaling within the ventral forebrain. Dev Dyn. 2022 Mar;251(3):459-480
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7382825
Gene symbol:	L1cam
Gene name:	L1 cell adhesion molecule

Antibody:	anti-L1CAM - C-terminal region (ARP63103_P050)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1A ©

Specimen 1A: embryonic day 17.5; Tg(mI56i-cre,-EGFP)1Kc/0 (more

)

Structure	Level	Pattern	Image	Note
TS25: cerebral internal capsule	Present		1A	Expressed in axons connecting the cortex to the thalamus.

1B ©

Specimen 1B: embryonic day 17.5; Mapk10^tm1Flv/Mapk10^tm1Flv, Mapk8^tm1Rjd/Mapk8^tm1Rjd, Mapk9^tm1Flv/Mapk9^tm1Flv, Tg(mI56i-cre,-EGFP)1Kc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present	Regionally restricted	1B	The internal capsule was not present in the mutant. A positive ectopic U-shaped bundle projected into the hypothalamus.

1C ©

Specimen 1C: embryonic day 17.5; Tg(mI56i-cre,-EGFP)1Kc/0 (more

)

Structure	Level	Pattern	Image	Note
TS25: anterior commissure	Present		1C	Crossed the midline.
TS25: corpus callosum	Present		1C	Crossed the midline.

1D ©

Specimen 1D: embryonic day 17.5; Mapk10^tm1Flv/Mapk10^tm1Flv, Mapk8^tm1Rjd/Mapk8^tm1Rjd, Mapk9^tm1Flv/Mapk9^tm1Flv, Tg(mI56i-cre,-EGFP)1Kc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: anterior commissure	Present		1D	Crossed the midline, similar as in the control.
TS25: corpus callosum	Present		1D	Crossed the midline, similar as in the control.

1E ©

Specimen 1E: embryonic day 17.5; Tg(mI56i-cre,-EGFP)1Kc/0 (more

)

Structure	Level	Pattern	Image	Note
TS25: cerebral internal capsule	Present		1E	Expressed in axons connecting the cortex to the thalamus.

1F ©

Specimen 1F: embryonic day 17.5; Mapk10^tm1Flv/Mapk10^tm1Flv, Mapk8^tm1Rjd/Mapk8^tm1Rjd, Mapk9^tm1Flv/Mapk9^tm1Flv, Tg(mI56i-cre,-EGFP)1Kc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: anterior commissure	Present		1F	Crossed the midline, similar as in the control.
TS25: corpus callosum	Present		1F	Crossed the midline, similar as in the control.

1I ©

1J ©

1L ©

Specimen 1I/J/L: embryonic day 15.5; Tg(mI56i-cre,-EGFP)1Kc/0 (more

)
Note: Double labeled: green - L1cam; red - Cntn2 (assay MGI:7382846).

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		1L , 1I , 1J

1N ©

1O ©

1Q ©

Specimen 1N/O/Q: embryonic day 15.5; Mapk10^tm1Flv/Mapk10^tm1Flv, Mapk8^tm1Rjd/Mapk8^tm1Rjd, Mapk9^tm1Flv/Mapk9^tm1Flv, Tg(mI56i-cre,-EGFP)1Kc/0 (more

)
Note: Conditional mutant. Double labeled: green - L1cam; red - Cntn2 (assay MGI:7382846).

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present	Regionally restricted	1Q , 1N , 1O	Positive axons were reduced in the mutant brain at the entry zone compared to controls. A population of L1cam+Cntn2- axons in the upper region were completely absent.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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