Ret In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:314923 Sunardi M, et al., Live visualization of a functional RET-EGFP chimeric receptor in homozygous knock-in mice. Dev Growth Differ. 2021 Aug;63(6):285-294
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7378443
Gene symbol:	Ret
Gene name:	ret proto-oncogene

Reporter:	GFP
	Direct Detection

Results

Specimen 3a: embryonic day 14.5; Ret^{tm6.1(RET/EGFP)Heno}/Ret^{tm6.1(RET/EGFP)Heno} (more

)
Note: Double labeled: green - Ret reporter; magenta - Uchl1 (assay MGI:7378375).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: enteric nervous system	enteric neuron	Present		3a''' , 3a , 3a'	Expression is in the cytoplasm and the plasma membrane of Uchl1-positive neurons.
TS22: midgut	enteric neuron	Present		3a''' , 3a , 3a'	Expression is in the cytoplasm and the plasma membrane of Uchl1-positive neurons.

Specimen 3b: embryonic day 13.5; Ret^{tm6.1(RET/EGFP)Heno}/Ret^{tm6.1(RET/EGFP)Heno} (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS21: enteric nervous system	enteric neuron	Present	Regionally restricted	3b	Expression is in enteric neural crest-derived cells at the migrating wavefront in the hindgut.
TS21: hindgut	enteric neuron	Present	Regionally restricted	3b	Expression is in enteric neural crest-derived cells at the migrating wavefront in the hindgut.

Specimen 3c: postnatal day 0; Ret^{tm6.1(RET/EGFP)Heno}/Ret^{tm6.1(RET/EGFP)Heno} (more

)
Note: Double labeled: green - Ret reporter; magenta - Nefm (assay MGI:7378444).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: sciatic nerve	motor neuron	Present	Punctate	3c left , 3c right	Expression is in a punctate pattern along the axons.

Specimen 3d: postnatal day 0; Ret^{tm6.1(RET/EGFP)Heno}/Ret^{tm6.1(RET/EGFP)Heno} (more

)
Note: Double labeled: green - Ret reporter; magenta - Th (assay MGI:7378445).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: sympathetic nervous system	sympathetic neuron	Present	Punctate	3d left , 3d right	Expression is in a punctate pattern along the axons.

Specimen 3e: embryonic day 13.5; Ret^{tm6.1(RET/EGFP)Heno}/Ret^{tm6.1(RET/EGFP)Heno} (more

)
Note: Double labeled: green - Ret reporter; magenta - Cdh1 (assay MGI:7378446).

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	3e right bottom , 3e left , 3e right top	Expression is at the apical membrane of the ureteric bud.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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