Gene Expression Data
Assay Details
Assay
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Reference:
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J:187631
Kredo-Russo S, et al., Pancreas-enriched miRNA refines endocrine cell differentiation. Development. 2012 Aug;139(16):3021-31
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7367243
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Gene symbol:
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Mir7-1
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Gene name:
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microRNA 7-1
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Probe:
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miR-7 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
S1B:
embryonic day 15.5
(more )
Specimen
S1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
|
Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas epithelium |
Present |
Clusters |
S1B
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Expression in a subset of epithelial cells, clustered within compartment of developing pancreas.
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Specimen
S1E:
embryonic day 13.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Ins1 (assay MGI:7367247) and Ins2 (assay MGI:7367279).
Specimen
S1E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
|
Type:
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section
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Embedding:
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Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Ins1 (assay MGI:7367247) and Ins2 (assay MGI:7367279).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: pancreas |
type B pancreatic cell |
Present |
Clusters |
S1E
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Expression in clustered cells, co-expressed with insulin.
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Specimen
S1F:
embryonic day 14.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Ins1 (assay MGI:7367247) and Ins2 (assay MGI:7367279).
Specimen
S1F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Embedding:
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Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Ins1 (assay MGI:7367247) and Ins2 (assay MGI:7367279).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
type B pancreatic cell |
Present |
Clusters |
S1F
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Expression in clustered cells, co-expressed with insulin.
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Specimen
S1G:
embryonic day 12.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; white - Neurog3 (assay MGI:7367248).
Specimen
S1G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; white - Neurog3 (assay MGI:7367248).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: pancreas epithelium |
progenitor cell of endocrine pancreas |
Trace |
Clusters |
S1G
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Expression detected at very low levels as early as E12.5 in a defined set of clustered epithelial cells, some of which co-express Neurog3.
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Specimen
S1J:
embryonic day 15.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Ghrl (assay MGI:7367249).
Specimen
S1J:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 15.5
|
Sex:
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Not Specified
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Type:
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section
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Ghrl (assay MGI:7367249).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
Regionally restricted |
S1J
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Expression in Ghrl-expressing cells.
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Specimen
1A,A':
embryonic day 15.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Ins1 (assay MGI:7367247) and Ins2 (assay MGI:7367279).
Specimen
1A,A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Ins1 (assay MGI:7367247) and Ins2 (assay MGI:7367279).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
type B pancreatic cell |
Present |
|
1A'
,
1A
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Specimen
1A,A'':
embryonic day 15.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; white - Gcg (assay MGI:7367252).
Specimen
1A,A'':
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; white - Gcg (assay MGI:7367252).
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Specimen
1B,B'':
embryonic day 15.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Cpa1 (assay MGI:7367253).
Specimen
1B,B'':
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Cpa1 (assay MGI:7367253).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
pancreatic acinar cell |
Absent |
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1B
,
1B''
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No expression in differentiated acinar cells.
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Specimen
1C,C',C'':
embryonic day 14.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Hnf1b (assay MGI:7367254).
Specimen
1C,C',C'':
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Hnf1b (assay MGI:7367254).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
pancreatic ductal cell |
Absent |
|
1C''
,
1C'
,
1C
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No expression in differentiated duct cells.
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Specimen
1D,D'':
embryonic day 13.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; white - Neurog3 (assay MGI:7367248).
Specimen
1D,D'':
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
|
Sex:
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Not Specified
|
Type:
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section
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; white - Neurog3 (assay MGI:7367248).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pancreas |
Present |
Regionally restricted |
1D
,
1D''
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Co-expression in many Neurog3-expressing cells.
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Specimen
1E,E'':
embryonic day 14.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; white - Neurog3 (assay MGI:7367248).
Specimen
1E,E'':
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; white - Neurog3 (assay MGI:7367248).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
Present |
Regionally restricted |
1E
,
1E''
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Co-expression in many Neurog3-expressing cells.
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Specimen
2E:
embryonic day 15.5
(more )
Note: Double labeled: red - Mir7-1 and Mir7-2; green - Pax6 (assay MGI:7367256).
Specimen
2E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Mir7-1 and Mir7-2; green - Pax6 (assay MGI:7367256).
|
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