Ncam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7346139
Gene symbol:	Ncam1
Gene name:	neural cell adhesion molecule 1

Antibody:	anti-NCAM [H28.123]
Detection system:	Secondary antibody

Results

1B left ©

1B middle ©

Specimen 1B: postnatal day 0 (more

)
Note: Cap mesenchyme stage. Triple labeled: green - Ncam1; red - Podxl2 (assay MGI:7346135); blue - Prkci (assay MGI:7346141).

Structure	Level	Pattern	Image	Note
TS27: cap mesenchyme	Present		1B right upper , 1B middle , 1B left	Expressed in the plasma membrane.

1C left ©

Specimen 1C: postnatal day 0 (more

)
Note: Pretubular aggregate stage. Double labeled: green - Ncam1; red - Podxl2 (assay MGI:7346135).

Structure	Level	Pattern	Image	Note
TS27: pretubular aggregate	Present		1C right middle , 1C left , 1C right merge	Expressed in the plasma membrane.

1D left ©

Specimen 1D: postnatal day 0 (more

)
Note: Renal vesicle stage. Double labeled: green - Ncam1; red - Podxl2 (assay MGI:7346135). Cyan - nuclear staining.

Structure	Level	Pattern	Image	Note
TS27: renal vesicle	Present		1D left , 1D right middle	Expression became restricted to the lateral surfaces.

5C left ©

Specimen 5C CTL: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi} (more

)
Note: Double labeled: green - Ncam1; red - Cd34 (assay MGI:7346202).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5C left	Expressed in the plasma membrane.

5C right ©

Specimen 5C cKO: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi}, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant. Double labeled: green - Ncam1; red - Cd34 (assay MGI:7346202).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5C right	Expressed in the plasma membrane, similar as in the control.

5D left ©

Specimen 5D CTL: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi} (more

)
Note: Double labeled: green - Ncam1; red - Pard6b (assay MGI:7346205).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5D left	Expressed in the plasma membrane.

5D right ©

Specimen 5D cKO: embryonic day 14.5; Podxl^tm1Parl/Podxl^tm1Parl, Podxl2^{tm1c(EUCOMM)Wtsi}/Podxl2^{tm1c(EUCOMM)Wtsi}, Tg(Pax3-cre)1Joe/0 (more

)
Note: Conditional mutant. Double labeled: green - Ncam1; red - Pard6b (assay MGI:7346205).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5D right	Expressed in the plasma membrane, similar as in the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/07/2024 MGI 6.23