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Gene Expression Data
Assay Details
Assay
Reference: J:327413 Gillnas S, et al., Severe cerebellar malformations in mutant mice demonstrate a role for PDGF-C/PDGFRalpha signalling in cerebellar development. Biol Open. 2022 Aug 15;11(8):bio059431
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7341422
Gene symbol: Podxl
Gene name: podocalyxin-like
Antibody: anti-Podxl (R&D; AF1556)
Detection system: Secondary antibody
Results
Specimen 4A: postnatal day 1; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present 4A Expression along ventricular zone of cerebellum.
Specimen 4B: postnatal day 1; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 4B No expression in ventricular zone closest to rhombic lip.
Specimen 4C,C': postnatal day 1; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 4C' , 4C Apical expression in cuboidal ependymal cells lined up side-by-side.
Specimen 4D,D': postnatal day 1; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 4D , 4D' Loss of expression in cells losing epithelial-lik morphology. Expression ended distinctly before reaching rhombic lip.
Specimen 5A: postnatal day 0; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: red - Podxl; white - betaIV tubulin (Tubb4a assay MGI:7341428).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present 5A
Specimen 5B: postnatal day 0; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: red - Podxl; white - betaIV tubulin (Tubb4a assay MGI:7341428).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 5B Expression lost in areas of denudation at fourth ventricle midline.
Specimen 5C: postnatal day 0; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: red - Podxl; white - S100b (assay MGI:7341429).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present 5C
Specimen 5D: postnatal day 0; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: red - Podxl; white - S100b (assay MGI:7341429).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 5D Expression lost in areas of denudation at fourth ventricle midline.
Specimen 5E: postnatal day 0; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: red - Podxl; green - Gfap (assay MGI:7341421).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present 5E
Specimen 5F: postnatal day 0; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: red - Podxl; green - Gfap (assay MGI:7341421).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 5F Expression lost in areas of denudation.
Specimen S2A,a: embryonic day 15.5; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS23: cerebellum ventricular layer Present Regionally restricted S2A , S2a Expression in apical side of ependymal cells.
Specimen S2B,b: embryonic day 15.5; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS23: cerebellum ventricular layer Present S2b , S2B
Specimen S2C,c: embryonic day 17.5; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS25: cerebellum ventricular layer Present Regionally restricted S2c , S2C Expression in apical side of ependymal cells.
Specimen S2D,d: embryonic day 17.5; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS25: cerebellum ventricular layer Present Regionally restricted S2D , S2d Expression did not cover whole ventricular zone.
Specimen S2E,e: postnatal day 0; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted S2e , S2E Expression in apical side of ependymal cells.
Specimen S2F,f: postnatal day 0; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted S2f , S2F Expression did not cover whole ventricular zone.
Specimen S2G,g: postnatal day 1; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted S2g , S2G Expression in apical side of ependymal cells.
Specimen S2H,h: postnatal day 1; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Triple labeled: red - Podxl; green - Cdh2 (assay MGI:7341430); white - Cx43 (Gja1 assay MGI:7341430).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted S2H , S2h Expression did not cover whole ventricular zone.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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