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Gene Expression Data
Assay Details
Assay
Reference: J:327413 Gillnas S, et al., Severe cerebellar malformations in mutant mice demonstrate a role for PDGF-C/PDGFRalpha signalling in cerebellar development. Biol Open. 2022 Aug 15;11(8):bio059431
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7341421
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: anti-GFAP (2.2B10) (13-0300)
Detection system: Secondary antibody
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-GFAP MGI:1314977 may have been used in this assay.
Results
Specimen 3G: postnatal day 7; Pdgfctm1Nagy/Pdgfc+, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Present 3G
Specimen 3H: postnatal day 7; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Present 3H
Specimen 3I': postnatal day 7; Pdgfctm1Nagy/Pdgfc+, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 3I' Evenly distributed cell nuclei and perpendicular Bergman glia in both rostral and caudal areas.
Specimen 3J': postnatal day 7; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 3J' Evenly distributed cell nuclei and perpendicular Bergman glia in both rostral and caudal areas.
Specimen 3K': postnatal day 7; Pdgfctm1Nagy/Pdgfc+, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 3K' Evenly distributed cell nuclei, but partly crooked Bergman glia in rostral area.
Specimen 3L': postnatal day 7; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 3L' Disorganised pattern of cell nuclei in caudal cerebellum with no perpendicular Bergman glia.
Specimen 5E,E': postnatal day 0; Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: green - Gfap; red - Podxl (assay MGI:7341422).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Absent 5E , 5E' No expression in ventricular zone.
Specimen 5F,F': postnatal day 0; Pdgfctm1Nagy/Pdgfctm1Nagy, Pdgfratm11(EGFP)Sor/Pdgfra+ (more )
Note: Double labeled: green - Gfap; red - Podxl (assay MGI:7341422).
Structure Level Pattern Image Note
TS27: cerebellum ventricular layer Present Regionally restricted 5F , 5F' Expression in areas where podocalyxin expression was lost in mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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