Antibody:	anti-PRG-5 (ab84659)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	2B	Expression throughout all cortical layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebral cortex	cerebral cortex neuron	Present	Regionally restricted	2B inset	Expression localized to soma and to dendrititic structures of cortical neurons

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	3A	Expression throughout brain.
TS28: cerebellum	Strong		3A
TS28: hippocampal formation	Strong		3A
TS28: main olfactory bulb	Strong		3A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1		Present	Regionally restricted	3B (merge) , 3B PRG5	Dendritic expression in hippocampal CA1.
TS28: hippocampus pyramidal cell layer	hippocampal pyramidal neuron	Present	Regionally restricted	3B (merge) , 3B PRG5	Expression in soma of pyramidal cells of stratum pyramidale.
TS28: hippocampus stratum lacunosum		Present	Regionally restricted	3B PRG5 , 3B (merge)	Expression in dendritic arbors in stratum radiale and stratum lacunosum moleculare.
TS28: hippocampus stratum radiatum		Present	Regionally restricted	3B PRG5 , 3B (merge)	Expression in dendritic arbors in stratum radiale and stratum lacunosum moleculare.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus		Weak	Regionally restricted	3C PRG5 , 3C (merge)	Low axonal expression in dentate gyrus.
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Strong	Regionally restricted	3C PRG5 , 3C (merge)	Strong expression in soma of granule cells in stratum granulosum.
TS28: dentate gyrus molecular layer		Present	Regionally restricted	3C PRG5 , 3C (merge)	Expression in basal dendritic arbors in molecular layer.
TS28: dentate gyrus hilus		Weak	Regionally restricted	3C (merge) , 3C PRG5	Low expression in mossy fibers in hilum.
TS28: dentate gyrus subgranular zone	neural progenitor cell	Absent		3C (merge) , 3C PRG5	No expression in neuronal stem cells in the subgranular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus		Weak	Regionally restricted	S2B PRG5 , S2B (merge)	Low axonal expression.
TS28: dentate gyrus granule cell layer		Strong		S2B PRG5 , S2B (merge)	Strong expression in stratum granulosum.
TS28: dentate gyrus molecular layer		Strong	Regionally restricted	S2B PRG5 , S2B (merge)	Strong expression in basal dendritic arbors in molecular layer.
TS28: dentate gyrus hilus		Weak	Regionally restricted	S2B PRG5 , S2B (merge)	Low expression in mossy fibers in hilum.
TS28: dentate gyrus subgranular zone	neural progenitor cell	Absent		S2B (merge) , S2B PRG5	No expression in neuronal stem cells in the subgranular zone.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Strong		S2C	Strong expression especially in inner plexiform layer and outer plexiform layer and mitral cells.
TS28: main olfactory bulb internal plexiform layer	Strong		S2C	Strong expression especially in inner plexiform layer and outer plexiform layer and mitral cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellar cortex	Purkinje cell	Strong	Regionally restricted	S2D	Strong expression in soma of Purkinje cells.
TS28: cerebellum molecular layer		Strong	Regionally restricted	S2D	Strong expression in dendritic arbors in molecular layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	Purkinje cell	Strong	Regionally restricted	3D (merge) , 3D PRG5	Strong expression in soma of Purkinje cells of cerebellum.
TS28: cerebellum molecular layer		Strong	Regionally restricted	3D PRG5 , 3D (merge)	Strong expression in dendritic arbors in molecular layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	cerebellar granule cell	Absent		3E PRG5 , 3E (merge)	No expression in granule cells of granule layer of cerebellum.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	dopaminergic neuron	Present		4A (merge) , 4A PRG5	Expression in dopaminergic neurosn in granule layer of main olfactory bulb.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	GABAergic interneuron	Present		4B PRG5 , 4B (merge)	Expression in inhibitory GABAergic interneurons in hippocampus.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	hippocampal astrocyte	Absent		4C (merge) , 4C PRG5	No expression in astrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus callosum	oligodendrocyte	Present	Regionally restricted	4D (merge) , 4D PRG5	Expression in a subtype of oligoedendrocytes. No expression in axonal fibers in the corpus callosum.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted	5A DPall	Expression in developing isocortex (dorsal pallium) not colocalized to Nes-expressing progenitor cells.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong	Regionally restricted	5A MPall	Particularly high abundance of expression in hippocampus (medial pallium) not colocalized to Nes-expressing progenitor cells.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present	Regionally restricted	5B (left)	Expression in subgranular zone not co-expressed with mitotic neurodifferentiation marker Nes.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present	Regionally restricted	5B (middle)	Expression in subgranular zone not co-expressed with mitotic neurodifferentiation marker Nes.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present	Regionally restricted	5B (right)	Expression in subgranular zone in late postmitotic phase co expressed with NeuN (Rbfox3).

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6_(montage)	Expression in later phase of neurodifferentiation.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6_(1)

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6_(2)

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6_(3)

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	6_(4)	Expression only at late stage of neurodifferentiation when neuroblasts have entered olfactory bulb and started differentiating.