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Gene Expression Data
Assay Details
Assay
Reference: J:326361 Li S, et al., Fate-mapping analysis of cochlear cells expressing Atoh1 mRNA via a new Atoh1(3*HA-P2A-Cre) knockin mouse strain. Dev Dyn. 2022 Jul;251(7):1156-1174
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7327506
Gene symbol: Atoh1
Gene name: atonal bHLH transcription factor 1
Reporter: HA
Antibody: anti-HA tag (clone 3F10)
Results
Specimen 2A,A': postnatal day 1; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlear inner hair cell Strong Graded 2A , 2A' Expression greater in hair cells of apical and middle turns that in basal turn.
TS27: cochlea cochlear outer hair cell Strong Graded 2A , 2A' Expression greater in hair cells of apical and middle turns that in basal turn.
Specimen 2B,B': postnatal day 1; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlear inner hair cell Present 2B' , 2B Expression greater in hair cells of apical and middle turns that in basal turn.
TS27: cochlea cochlear outer hair cell Present 2B' , 2B Expression greater in hair cells of apical and middle turns that in basal turn.
Specimen 2C,C': postnatal day 1; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlear outer hair cell Trace 2C , 2C'
TS27: cochlea cochlear inner hair cell Weak 2C' , 2C
Specimen 2D,D': postnatal day 1; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Sox2 (assay MGI:7327508).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlear inner hair cell Present 2D' , 2D
TS27: cochlea cochlear outer hair cell Present 2D' , 2D
TS27: cochlea supporting cell of cochlea Absent 2D' , 2D
Specimen 2E,E': postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear outer hair cell Trace 2E , 2E' Expression level markedly lower in hair cells at p5 than at P1.
TS28: cochlea cochlear inner hair cell Weak 2E , 2E' Expression in inner hair cells higher than in outer hair cells/
Specimen 2F,F': postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear outer hair cell Trace 2F' , 2F Expression level markedly lower in hair cells at p5 than at P1.
TS28: cochlea cochlear inner hair cell Weak 2F' , 2F Expression in inner hair cells higher than in outer hair cells/
Specimen 2G,G' OHC: postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear outer hair cell Ambiguous 2G OHC , 2G' OHC Expression level markedly lower in hair cells at p5 than at P1.
Specimen 2G,G' IHC: postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Trace 2G' IHC , 2G IHC Expression level markedly lower in hair cells at p5 than at P1.
Specimen 2H,H': postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Sox2 (assay MGI:7327508).
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlear inner hair cell Ambiguous 2H , 2H' Expression almost undetectable at P10.
TS28: cochlea cochlear outer hair cell Ambiguous 2H , 2H' Expression almost undetectable at P10.
TS28: cochlea supporting cell of cochlea Absent 2H' , 2H
Specimen 3A',A'': postnatal day 1; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS27: utricle auditory epithelial supporting cell Present Single cells 3A'' , 3A'
TS27: utricle auditory hair cell Present Single cells 3A'' , 3A'
Specimen 3B',B'': postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Level Pattern Image Note
TS28: crista ampullaris Present Regionally restricted 3B' , 3B''
TS28: utricle Present Regionally restricted 3B' , 3B''
Specimen 3C',C'': postnatal day 5; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS28: utricle auditory epithelial supporting cell Present Single cells 3C' , 3C''
Specimen 3D',D'': postnatal day 15; Atoh1em1(cre)Zyliu/Atoh1+ (more )
Note: Double labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507).
Structure Cell Type Level Pattern Image Note
TS28: utricle auditory epithelial supporting cell Present Single cells 3D'' , 3D'
Specimen 5A'',A''': embryonic day 12.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS20: saccule Present 5A'' , 5A''' Expression in vestibular sensory regions.
TS20: utricle Present 5A'' , 5A''' Expression in vestibular sensory regions.
Specimen 5B'',B''': embryonic day 12.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS20: cochlea Absent 5B'' , 5B'''
Specimen 5C: embryonic day 13.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS21: saccule epithelium Present 5C Expression in vestibular sensory epithelium.
TS21: utricle epithelium Present 5C Expression in vestibular sensory epithelium.
TS21: cochlea epithelium Absent 5C No expression in cochlear sensory epithelium.
Specimen 5D'',D''': embryonic day 13.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS21: saccule auditory epithelial supporting cell Present Single cells 5D'' , 5D'''
Specimen 5E'',E''': embryonic day 13.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS21: utricle auditory epithelial supporting cell Present Single cells 5E''' , 5E''
Specimen 6A'',A''': embryonic day 14.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS22: cochlea Ambiguous Regionally restricted 6A'' , 6A'''
Specimen 6B'',B''': embryonic day 14.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS22: cochlea Ambiguous 6B'' , 6B'''
Specimen 6C'',C''': embryonic day 14.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS22: cochlea Weak Regionally restricted 6C'' , 6C'''
Specimen 6D'',D''': embryonic day 14.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS22: cochlea cochlear outer hair cell Present Single cells 6D'' , 6D'''
TS22: cochlea cochlear inner hair cell Present 6D''' , 6D''
Specimen 7B'',B''': embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS23: cochlea cochlear inner hair cell Trace 7B'' , 7B'''
Specimen 7C'',C''': embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS23: cochlea cochlear outer hair cell Present 7C''' , 7C''
TS23: cochlea cochlear inner hair cell Strong 7C''' , 7C''
Specimen 7D'',D''': embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS23: cochlea cochlear outer hair cell Present 7D'' , 7D'''
TS23: cochlea cochlear inner hair cell Strong 7D''' , 7D''
Specimen 7G: embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS23: cochlea Ambiguous 7G
Specimen 7H'',H''': embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS23: cochlea Ambiguous 7H''' , 7H''
Specimen 7I'',I''': embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Level Pattern Image Note
TS23: cochlea Weak Single cells 7I''' , 7I''
Specimen 7J'',J''': embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Atoh1 reporter; green - Myo6 (assay MGI:7327507); red - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS23: cochlea cochlear inner hair cell Present Single cells 7J''' , 7J''
Specimen E13.5 not shown: embryonic day 13.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: cochlear ganglion spiral ganglion neuron Absent
Specimen E15.5 not shown: embryonic day 15.5; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS23: cochlear ganglion spiral ganglion neuron Absent
Specimen P1 not shown: postnatal day 1; Atoh1em1(cre)Zyliu/Atoh1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: cochlear ganglion spiral ganglion neuron Absent
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